Proteomic analysis of low-abundant integral plasma membrane proteins based on gels

被引：53

作者：

Zhang, L. -J. ^{[1
]}

Wang, X. -e ^{[1
]}

Peng, X. ^{[1
]}

Wei, Y. -J. ^{[1
]}

Cao, R. ^{[1
]}

Liu, Z. ^{[1
]}

Xiong, J. -X. ^{[1
]}

Yin, X. -f. ^{[1
]}

Ping, C. ^{[1
]}

Liang, S. ^{[1
]}

机构：

[1] Hunan Normal Univ, Key Lab Prot Chem & Dev Biol, Natl Educ Comm, Coll Life Sci, Changsha 410081, Peoples R China

来源：

CELLULAR AND MOLECULAR LIFE SCIENCES | 2006年 / 63卷 / 15期

关键词：

rat liver; plasma membrane; SDS-gel electrophoresis; microdomain;

D O I：

10.1007/s00018-006-6126-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

To characterize low-copy integral membrane proteins and offer some methods for human liver proteome projects, we fractionated highly purified rat liver plasma membrane (PM). PM was purified through two sucrose density gradient centrifugations, and treated with 0.1 M Na2CO3, chloroform/methanol and Triton X-100. Proteins were separated by electrophoresis and submitted to mass spectrometry analysis. Four hundred and fiftyseven non-redundant membrane proteins were identified, of which 23% (105) were integral membrane proteins with one or more transmembrane domains. One hundred and fifty-three (33.5%) had no location annotation and 68 were unknown-function proteins. The proteins from different fractions were complementory. A database search for all identified proteins revealed that 53 proteins were involved in the cell communication pathway. More interestingly, more than 50% of the proteins had a protein abundance index concentration of less than 0.1 mol/l, and 12% proteins a concentration 100 times less than that of arginase 1 and actin.

引用

页码：1790 / 1804

页数：15

共 44 条

[1]

Comprehensive proteomic analysis of breast cancer cell membranes reveals unique proteins with potential roles in clinical cancer [J].