TLC analysis of formaldehyde produced by metabolic N-demethylation

被引：13

作者：

Kalász, H

Szarvas, T

Szarkane-Bolehovszky, A

Lengyel, J

机构：

[1] Semmelweis Univ, Dept Pharmacol & Pharmacotherapy, H-1089 Budapest, Hungary

[2] Semmelweis Univ, Cent Isotope Lab, H-1089 Budapest, Hungary

[3] Inst Isotopes Co Ltd, Budapest, Hungary

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2002年 / 25卷 / 10-11期

关键词：

formaldehyde; TLC; thin layer chromatography; (-)-deprenyl; microsomal metabolism;

D O I：

10.1081/JLC-120005706

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Formaldehyde production was observed during the rat liver microsomal metabolism of (-)-deprenyl. Formaldehyde was reacted to its dimedone adduct. The formaldemethone was identified using thin-layer chromatography, combined with X-ray film autoradiography. Standard samples served for the reliable identification of spots of formaldemethone and those of the other metabolites.

引用

页码：1589 / 1598

页数：10