Factors influencing cDNA microarray hybridization on silylated glass slides

被引：23

作者：

Franssen-val Hal, NLW

Vorst, O

Kramer, E

Hall, RD

Keijer, J

机构：

[1] State Inst Qual Control Agr Prod, Gene Express & Detect Ctr, NL-6700 AE Wageningen, Netherlands

[2] Plant Res Int, Gene Express & Detect Ctr, Business Unit Cell Cybernet, NL-6700 AA Wageningen, Netherlands

来源：

ANALYTICAL BIOCHEMISTRY | 2002年 / 308卷 / 01期

关键词：

microarray; gene expression; hybridization; silylated slides; technological aspects;

D O I：

10.1016/S0003-2697(02)00203-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

cDNA microarray technology is becoming the technique of choice for studying gene expression and gene expression patterns. Although experimental protocols are available, only limited methodological information on microarray manufacture, hybridization, and signal interpretation has been published. The aim of this paper is to provide more insight into the practical aspects of microarray construction and hybridization. The influence of the size, composition, and concentration of the spotted DNA fragments on the final hybridization signal and the effect of hybridization volume, sample concentration, and sample depletion have been tested and are discussed. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：5 / 17

页数：13

共 25 条

[1] Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms [J].