Bio-sniffers for ethanol and acetaldehyde using carbon and Ag/AgCl coated electrodes

被引：15

作者：

Gessei, Tomoko ^{[2
,3
]}

Sato, Hitoshi ^{[4
]}

Kazawa, Elito ^{[2
,3
]}

Kudo, Hiroyuki ^{[1
]}

Saito, Hirokazu ^{[1
]}

Mitsubayashi, Kohji ^{[1
]}

机构：

[1] Tokyo Med & Dent Univ, Inst Biomat & Bioengn, Chiyoda Ku, Tokyo, Japan

[2] Tokyo Metropolitan Ind Technol Res Inst, Kita Ku, Tokyo 1158586, Japan

[3] Tokyo Med & Dent Univ, Grad Sch Med & Dent, Bunkyo Ku, Tokyo, Japan

[4] Tokai Univ, Dept Human & Informat Sci, Kanagawa 2591100, Japan

来源：

MICROCHIMICA ACTA | 2009年 / 165卷 / 1-2期

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

Breath analysis; Alcohol metabolic; Gas monitoring; Biosensor; Ethanol; Acetaldehyde; EXHALED HYDROGEN-PEROXIDE; ALCOHOL; BIOSENSORS; BLOOD; BREATH; OXIDASE; VAPOR;

D O I：

10.1007/s00604-008-0117-z

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Monitoring of ethanol and acetaldehyde in expired gas after drinking is an effective method for assessment of alcohol metabolic function. We have developed bioelectronic gas sensors (bio-sniffers) for convenient measurement of ethanol and acetaldehyde. The bio-sniffers were fabricated using a simple process and inexpensive method. The process consisted of coating carbon and Ag/AgCl on a filter paper using screen-printing, and immobilizing alcohol oxidase and aldehyde dehydrogenase on the carbon electrode. These bio-sniffers showed good response to ethanol and acetaldehyde vapor and were used to sense these vapors in the concentration range 1.0 to 100 ppm and 0.2 to 4.0 ppm, respectively. The calibration ranges cover the alcohol and acetaldehyde concentration in breath air after drinking. The bio-sniffer for acetaldehyde is applicable also to evaluate other aldehydes (i.e. formaldehyde as volatile organic compounds).

引用

页码：179 / 186

页数：8

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