133 new gene localizations on the rabbit cytogenetic map

被引：13

作者：

Chantry-Darmon, C

Rogel-Gaillard, C

Bertaud, M

Urien, C

Perrocheau, M

Chardon, P

Hayes, H

机构：

[1] INRA, Lab Genet Biochim & Cytogenet, F-78350 Jouy En Josas, France

[2] INRA, CEA, UMR 13 314, Lab Radiobiol & Etud Genome, F-78350 Jouy En Josas, France

来源：

CYTOGENETIC AND GENOME RESEARCH | 2003年 / 103卷 / 1-2期

关键词：

D O I：

10.1159/000076310

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Rabbit (Oryctolagus cuniculus), besides its interest for medical research and biotechnological applications, has a small agronomic production in southern European countries. However, it is still a "map-poor" species with about 80 genes mapped. Recently, useful tools for research on this species have been developed, such as heterologous human-rabbit chromosome painting data and a rabbit BAC library. In this study, our aim is to enrich the rabbit cytogenetic map using the FISH technique. Towards this, we have used cDNAs (rabbit and non rabbit) present in the public databases to determine intra-exon primers used to screen our three-genome equivalent BAC library, by standard PCR directly on DNA pools, and by hybridization of high-density filters. 133 BAC clones containing the genes of interest were isolated and FISH-mapped to the rabbit chromosomes. We present the localization of new genes on all rabbit chromosomes except OCU20 and OCUY and some preliminary data on the rabbit/human comparative map. In addition, this set of BAC clones quite regularly distributed on the rabbit genome will be useful to isolate microsatellites, in order to construct a first generation genetic map. Copyright (C) 2003 S. Karger AG, Basel

引用

页码：192 / 201

页数：10

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