Structure and in Vivo Requirement of the Yeast Spt6 SH2 Domain

被引:39
作者
Dengl, Stefan
Mayer, Andreas
Sun, Mai
Cramer, Patrick [1 ]
机构
[1] Univ Munich, Gene Ctr, D-81377 Munich, Germany
关键词
Cellular signaling; RNA polymerase II; chromatin transcription; nucleosome assembly; evolution of SH2 domains; RNA-POLYMERASE-II; TRANSCRIPTION ELONGATION-FACTORS; SACCHAROMYCES-CEREVISIAE; CRYSTAL-STRUCTURE; DIFFRACTION DATA; GENE ONTOLOGY; PROTEIN; COMPLEXES; ALIGNMENT; DNA;
D O I
10.1016/j.jmb.2009.04.016
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
During transcription elongation through chromatin, the Ser2-phosphorylated C-terminal repeat domain of RNA polymerase II binds the C-terminal Src homology 2 (SH2) domain of the nucleosome re-assembly factor Spt6. This SH2 domain is unusual in its specificity to bind phosphoserine, rather than phosphotyrosine and because it is the only SH2 domain in the yeast genome. Here, we report the high-resolution crystal structure of the SH2 domain from Candida glabrata Spt6. The structure combines features from both structural subfamilies of SH2 domains, suggesting it resembles a common ancestor of all SH2 domains. Two conserved surface pockets deviate from those of canonical SH2 domains, and may explain the unusual phosphoserine specificity. Differential gene expression analysis reveals that the SH2 domain is required for normal expression of a subset of yeast genes, and is consistent with Multiple functions of Spt6 in chromatin transcription. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:211 / 225
页数:15
相关论文
共 70 条
[1]   Transcriptional activators are dispensable for transcription in the absence of Spt6-mediated chromatin reassembly of promoter regions [J].
Adkins, MW ;
Tyler, JK .
MOLECULAR CELL, 2006, 21 (03) :405-416
[2]   High-resolution localization of Drosophila Spt5 and Spt6 at heat shock genes in vivo:: roles in promoter proximal pausing and transcription elongation [J].
Andrulis, ED ;
Guzmán, E ;
Döring, P ;
Werner, J ;
Lis, JT .
GENES & DEVELOPMENT, 2000, 14 (20) :2635-2649
[3]  
ARDEHALI MB, 2009, EMBO J 0312
[4]   Gene Ontology: tool for the unification of biology [J].
Ashburner, M ;
Ball, CA ;
Blake, JA ;
Botstein, D ;
Butler, H ;
Cherry, JM ;
Davis, AP ;
Dolinski, K ;
Dwight, SS ;
Eppig, JT ;
Harris, MA ;
Hill, DP ;
Issel-Tarver, L ;
Kasarskis, A ;
Lewis, S ;
Matese, JC ;
Richardson, JE ;
Ringwald, M ;
Rubin, GM ;
Sherlock, G .
NATURE GENETICS, 2000, 25 (01) :25-29
[5]   Three-dimensional structure of the Stat3β homodimer bound to DNA [J].
Becker, S ;
Groner, B ;
Müller, CW .
NATURE, 1998, 394 (6689) :145-151
[6]   CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].
BENJAMINI, Y ;
HOCHBERG, Y .
JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300
[7]   The SWISS-PROT protein knowledgebase and its supplement TrEMBL in 2003 [J].
Boeckmann, B ;
Bairoch, A ;
Apweiler, R ;
Blatter, MC ;
Estreicher, A ;
Gasteiger, E ;
Martin, MJ ;
Michoud, K ;
O'Donovan, C ;
Phan, I ;
Pilbout, S ;
Schneider, M .
NUCLEIC ACIDS RESEARCH, 2003, 31 (01) :365-370
[8]   Evidence that Spt6p controls chromatin structure by a direct interaction with histones [J].
Bortvin, A ;
Winston, F .
SCIENCE, 1996, 272 (5267) :1473-1476
[9]   Crystallography & NMR system:: A new software suite for macromolecular structure determination [J].
Brunger, AT ;
Adams, PD ;
Clore, GM ;
DeLano, WL ;
Gros, P ;
Grosse-Kunstleve, RW ;
Jiang, JS ;
Kuszewski, J ;
Nilges, M ;
Pannu, NS ;
Read, RJ ;
Rice, LM ;
Simonson, T ;
Warren, GL .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 :905-921
[10]  
BUDISA N, 1995, EUR J BIOCHEM, V230, P788