Th GacS/GacA signal transduction system of Pseudomonas aeruginosa acts exclusively through its control over the transcription of the RsmY and RsmZ regulatory small RNAs

被引:277
作者
Brencic, Anja [1 ]
McFarland, Kirsty A. [2 ]
McManus, Heather R. [2 ]
Castang, Sandra [2 ]
Mogno, Ilaria [3 ]
Dove, Simon L. [2 ]
Lory, Stephen [1 ]
机构
[1] Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA
[2] Harvard Univ, Sch Med, Div Infect Dis, Childrens Hosp, Boston, MA 02115 USA
[3] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA
基金
美国国家卫生研究院;
关键词
ENTERICA SEROVAR TYPHIMURIUM; EXTRACELLULAR PROTEINS; OPPORTUNISTIC PATHOGEN; FLUORESCENS CHA0; VIRULENCE GENES; IDENTIFICATION; EXPRESSION; SALMONELLA; CAROTOVORA; INFECTION;
D O I
10.1111/j.1365-2958.2009.06782.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report here the results of an analysis of the regulatory range of the GacS/GacA two-component system in Pseudomonas aeruginosa. Using microarrays, we identified a large number of genes that are regulated by the system, and detected a near complete overlap of these genes with those regulated by two small RNAs (sRNAs), RsmY and RsmZ, suggesting that the expression of all GacA-regulated genes is RsmY/Z-dependent. Using genome-wide DNA-protein interaction analyses, we identified only two genomic regions that associated specifically with GacA, located upstream of the rsmY and rsmZ genes. These results demonstrate that in P. aeruginosa, the GacS/GacA system transduces the regulatory signals to downstream genes exclusively by directly controlling the expression of only two genes rsmY and rsmZ. These two sRNAs serve as intermediates between the input signals and the output at the level of mRNA stability, although additional regulatory inputs can influence the levels of these two riboregulators. We show that the A+T-rich DNA segment upstream of rsmZ is bound and silenced by MvaT and MvaU, the global gene regulators of the H-NS family. This work highlights the importance of post-transcriptional mechanisms involving sRNAs in controlling gene expression during bacterial adaptation to different environments.
引用
收藏
页码:434 / 445
页数:12
相关论文
共 32 条
[1]   Identification of bacterial small non-coding RNAs: experimental approaches [J].
Altuvia, Shoshy .
CURRENT OPINION IN MICROBIOLOGY, 2007, 10 (03) :257-261
[2]   CsrB sRNA family: sequestration of RNA-binding regulatory proteins [J].
Babitzke, Paul ;
Romeo, Tony .
CURRENT OPINION IN MICROBIOLOGY, 2007, 10 (02) :156-163
[3]   Determination of the regulon and identification of novel mRNA targets of Pseudomonas aeruginosa RsmA [J].
Brencic, Anja ;
Lory, Stephen .
MOLECULAR MICROBIOLOGY, 2009, 72 (03) :612-632
[4]   Influence of the regulatory protein RsmA on cellular functions in Pseudomonas aeurginosa PAO1, as revealed by transcriptome analysis [J].
Burrowes, E ;
Baysse, C ;
Adams, C ;
O'Gara, F .
MICROBIOLOGY-SGM, 2006, 152 :405-418
[5]   H-NS family members function coordinately in an opportunistic pathogen [J].
Castang, Sandra ;
McManus, Heather R. ;
Turner, Keith H. ;
Dove, Simon L. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (48) :18947-18952
[6]   Regulatory network controlling extracellular proteins in Erwinia carotovora subsp carotovora:: FlhDC, the master regulator of flagellar genes, activates rsmB regulatory RNA production by affecting gacA and hexA (lrhA) expression [J].
Cui, Yaya ;
Chatterjee, Asita ;
Yang, Hailian ;
Chatterjee, Arun K. .
JOURNAL OF BACTERIOLOGY, 2008, 190 (13) :4610-4623
[7]   Identification of CsrC and characterization of its role in epithelial cell invasion in Salmonella enterica serovar Typhimurium [J].
Fortune, DR ;
Suyemoto, M ;
Altier, C .
INFECTION AND IMMUNITY, 2006, 74 (01) :331-339
[8]   Bacterial response regulators: versatile regulatory strategies from common domains [J].
Gao, Rong ;
Mack, Timothy R. ;
Stock, Ann M. .
TRENDS IN BIOCHEMICAL SCIENCES, 2007, 32 (05) :225-234
[9]   A signaling network reciprocally regulates genes associated with acute infection and chronic persistence in Pseudomonas aeruginosa [J].
Goodman, AL ;
Kulasekara, B ;
Rietsch, A ;
Boyd, D ;
Smith, RS ;
Lory, S .
DEVELOPMENTAL CELL, 2004, 7 (05) :745-754
[10]   Direct interaction between sensor kinase proteins mediates acute and chronic disease phenotypes in a bacterial pathogen [J].
Goodman, Andrew L. ;
Merighi, Massimo ;
Hyodo, Mamoru ;
Ventre, Isabelle ;
Filloux, Alain ;
Lory, Stephen .
GENES & DEVELOPMENT, 2009, 23 (02) :249-259