Matrix Gene Expression in Dermal Fibroblasts Cultured on Hyaluronan-coated Polysulfone Membranes

被引：6

作者：

Attia, J. ^{[1
]}

Boumediene, K. ^{[1
]}

Pujol, J. P. ^{[1
]}

Valleton, J. M. ^{[2
]}

Huet, E. ^{[3
]}

Nguyen, Q. T. ^{[4
]}

机构：

[1] Univ Caen, Lab Extracellular Matrix & Pathol, Fac Med, ICORE,IFR 146, F-14032 Caen, France

[2] Univ Rouen, CNRS, UMR 6522, F-76821 Mont St Aignan, France

[3] Univ Paris 12, CNRS, Lab CRRET, Sci UFR,UMR 7149, F-94010 Creteil, France

[4] Univ Rouen, Lab Polymers, UFR Sci, Surfaces FRE 3101, F-76821 Mont St Aignan, France

来源：

JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS | 2009年 / 24卷 / 04期

关键词：

dermal fibroblasts; polysulfone membrane; coated polysulfone membrane; hyaluronan; hyaluronic acid; matrix gene expression; wound healing; nonscarring healing; MMP1; enhanced MMP3 and MMP2; BY-LAYER DEPOSITION; FUNCTIONAL-DIFFERENTIATION; PROLIFERATION; ADHESION; METALLOPROTEINASES; KERATINOCYTES; ASSOCIATION; CULTIVATION; COLLAGENASE; TRANSITION;

D O I：

10.1177/0883911509105847

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Polysulfone (PSU) membranes, coated and uncoated hyaluronan (HA), were compared for their ability to allow dermal fibroblast express genes related to extracellular matrix synthesis and remodeling. Fibroblasts type I and type III collagens were studied on both types of membranes; only type I collagen was synthesized on control cultures in plastic Petri dishes, whereas type III collagen was also expressed on PSU membranes. Expression of metalloproteinase (MMP)1, MMP3, and MMP2 was enhanced on PSU and HA-coated PSU membranes, with a lower level of MMP2 on HA-covered membranes. These membranes promote fetal- like matrices that provide good support for skin wound healing as well as favor nonscarring tissue repair.

引用

页码：329 / 349

页数：21

共 45 条

[1]

COLLAGENASE IN WOUND-HEALING - EFFECT OF WOUND AGE AND TYPE [J].

AGREN, MS ;

TAPLIN, CJ ;

WOESSNER, JF ;

EAGLSTEIN, WH ;

MERTZ, PM .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1992, 99 (06) :709-714

[2]

Review. Hyaluronan: A powerful tissue engineering tool [J].

Allison, David D. ;

Grande-Allen, K. Jane .

TISSUE ENGINEERING, 2006, 12 (08) :2131-2140

[3]

Evaluation of Adhesion, Proliferation, and Functional Differentiation of Dermal Fibroblasts on Glycosaminoglycan-Coated Polysulfone Membranes [J].

Attia, Joan ;

Legendre, Florence ;

Nguyen, Quang Trong ;

Bauge, Catherine ;

Boumediene, Karim ;

Pujol, Jean Pierre .

TISSUE ENGINEERING PART A, 2008, 14 (10) :1687-1697

[4]

Bourguignon LYW, 1998, J CELL PHYSIOL, V176, P206, DOI 10.1002/(SICI)1097-4652(199807)176:1<206::AID-JCP22>3.0.CO

[5]

2-3

[6]

Impaired wound contraction in stromelysin-1-deficient mice [J].

Bullard, KM ;

Lund, L ;

Mudgett, JS ;

Mellin, TN ;

Hunt, TK ;

Murphy, B ;

Ronan, J ;

Werb, Z ;

Banda, MJ .

ANNALS OF SURGERY, 1999, 230 (02) :260-265

[7]

Biodegradable elastomeric polyurethane membranes as chondrocyte carriers for cartilage repair [J].

Chia, Shi-Lu ;

Gorna, Katarzyna ;

Gogolewski, Sylwester ;

Alini, Mauro .

TISSUE ENGINEERING, 2006, 12 (07) :1945-1953

[8]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[9]

Dynamic study of the transition from hyaluronan- to integrin-mediated adhesion in chondrocytes [J].

Cohen, M ;

Kam, Z ;

Addadi, L ;

Geiger, B .

EMBO JOURNAL, 2006, 25 (02) :302-311

[10]

A blank slate? Layer-by-layer deposition of hyaluronic acid and chitosan onto various surfaces [J].

Croll, Tristan I. ;

O'Connor, Andrea J. ;

Stevens, Geoffrey W. ;

Cooper-White, Justin J. .

BIOMACROMOLECULES, 2006, 7 (05) :1610-1622

← 1 2 3 4 5 →