Plant retinoblastoma homologues control nuclear proliferation in the female gametophyte

被引:199
作者
Ebel, C [1 ]
Mariconti, L [1 ]
Gruissem, W [1 ]
机构
[1] Swiss Fed Inst Technol, Swiss Fed Inst Technol, Inst Plant Sci, CH-8092 Zurich, Switzerland
基金
加拿大健康研究院; 美国国家卫生研究院;
关键词
D O I
10.1038/nature02637
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Haploid spores of plants divide mitotically to form multicellular gametophytes. The female spore ( megaspore) of most flowering plants develops by means of a well-defined programme into the mature megagametophyte consisting of the egg apparatus and a central cell(1,2). We investigated the role of the Arabidopsis retino-blastoma(3,4) protein homologue and its function as a negative regulator of cell proliferation during megagametophyte development. Here we show that three mutant alleles of the gene for the Arabidopsis retinoblastoma-related protein, RBR1 (ref. 4), are gametophytic lethal. In heterozygous plants 50% of the ovules are aborted when the mutant allele is maternally inherited. The mature unfertilized mutant megagametophyte fails to arrest mitosis and undergoes excessive nuclear proliferation in the embryo sac. Supernumerary nuclei are present at the micropylar end of the megagametophyte, which develops into the egg apparatus and central cell. The central cell nucleus, which gives rise to the endosperm after fertilization, initiates autonomous endosperm development reminiscent of fertilization-independent seed (fis) mutants(5). Thus, RBR1 has a novel and previously unrecognized function in cell cycle control during gametogenesis and in the repression of autonomous endosperm development.
引用
收藏
页码:776 / 780
页数:5
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