Otic Mesenchyme Expression of Cre Recombinase Directed by the Inner Ear Enhancer of the Brn4/Pou3f4 Gene

被引：17

作者：

Ann, Kyung J. ^{[1
]}

Passero, Frank, Jr. ^{[1
]}

Crenshaw, E. Bryan, III ^{[1
,2
,3
]}

机构：

[1] Childrens Hosp Philadelphia, Ctr Childhood Commun, Mammalian Neurogenet Grp, Div Pediat Otolaryngol, Philadelphia, PA 19104 USA

[2] Univ Penn, Sch Med, Dept Otorhinolaryngol Head & Neck Surg, Philadelphia, PA 19104 USA

[3] Univ Penn, Sch Med, Neurosci Grad Grp, Philadelphia, PA 19104 USA

来源：

GENESIS | 2009年 / 47卷 / 03期

关键词：

inner ear; Cre recombinase; conditional knockout; otic mesenchyme; Pou3f4; enhancer region; LACZ EXPRESSION; DEAFNESS; DFN3;

D O I：

10.1002/dvg.20454

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Brn4/Pou3f4 is a POU-domain transcription factor expressed in the otic mesenchyme that is required for the normal development of the inner ear. In this report, we describe the isolation of an otic mesenchyme enhancer in the Brn4 gene. Subsequently, this enhancer was used to drive the expression of Cre recombinase in the otic mesenchyme of transgenic mice. When intercrossed with the ROSA reporter strain, R26R, beta-galactosidase expression is detected in several inner ear structures derived from otic mesenchyme, including the temporal bone, spiral ligament, spiral limbus, and mesenchyme underlying sensory epithelium of the utricle, saccule and semicircular canals. Thus, this Cre pedigree can induce conditional rearrangement of genes in the otic mesenchyme, and will serve as a powerful genetic tool to characterize the function of genes in the mesenchymal tissues of the inner ear. genesis 47:137-141, 2009. (c) 2009 Wiley-Liss, Inc.

引用

页码：137 / 141

页数：5

共 12 条

[1]

Ahn K, 2001, DEVELOPMENT, V128, P4449

[2] FURTHER MUTATIONS IN BRAIN-4 (POU3F4) CLARIFY THE PHENOTYPE IN THE X-LINKED DEAFNESS, DFN3 [J].