Tomato Prf is a member of the leucine-rich repeat class of plant disease resistance genes and lies embedded within the Pto kinase gene cluster

被引：443

作者：

Salmeron, JM

Oldroyd, GED

Rommens, CMT

Scofield, SR

Kim, HS

Lavelle, DT

Dahlbeck, D

Staskawicz, BJ

机构：

[1] UNIV CALIF BERKELEY, DEPT PLANT BIOL, BERKELEY, CA 94720 USA

[2] CIBA GEIGY CORP, AGR BIOTECHNOL, RES TRIANGLE PK, NC 27709 USA

[3] NATL SCI FDN, CTR ENGN PLANTS RESISTANCE AGAINST PATHOGENS, DAVIS, CA 95616 USA

来源：

CELL | 1996年 / 86卷 / 01期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/S0092-8674(00)80083-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In tomato, resistance to Pseudomonas syringae pv. tomato (Pst) strains expressing the avirulence gene avrPto requires the presence of at least two host genes, designated Pto and Prf. Here we report that Prf encodes a protein with leucine-zipper, nucleotide-binding, and leucine-rich repeat motifs, as are found in a number of resistance gene products from other plants. prf mutant alleles (4) were found to carry alterations within the Prf coding sequence. A genomic fragment containing Prf complemented a prf mutant tomato line both for resistance to Pst strains expressing avrPto and for sensitivity to the insecticide Fenthion. Prf resides in the middle of the Pto gene cluster, 24 kb from the Pto gene and 500 bp from the Fen gene.

引用

页码：123 / 133

页数：11