Routine determination of flumequine in kidney tissue of pig using automated liquid chromatography

被引：9

作者：

Guyonnet, J

Pacaud, M

Richard, M

Doisi, A

Spavone, F

Hellings, P

机构：

[1] Dept. Pharmacokin. and Drug Metab., Sanofi S. Nutrition Animale

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1996年 / 679卷 / 1-2期

关键词：

flumequine; hydroxyflumequine;

D O I：

10.1016/0378-4347(95)00582-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic assay is described as a routine analytical method for the determination of flumequine (FLU) and its hydroxylated metabolite (OH-FLU) in pig kidney tissue. Kidney samples (2 g) containing FLU and OH-FLU were extracted by liquid-liquid extraction with ethyl acetate (10 mi). Analytical separations were performed by reversed-phase HPLC with fluorometric detection at 252 nm excitation and 356 nm emission under gradient conditions. The mobile phase was acetonitrile-2.7 . 10(-3) M oxalic acid in water (pH 2.5). The assay is specific and reproducible within the flumequine range of 0.050-2.5 mu g/g and recovery at 0.050 mu g/g was 94.8%.

引用

页码：177 / 184

页数：8