Plicatamide: A lead to the biosynthetic origins of the tunichromes?

被引：22

作者：

Tincu, JA

Craig, AG

Taylor, SW ^{[1
]}

机构：

[1] Univ Calif San Diego, Scripps Inst Oceanog, Ctr Marine Biotechnol & Biomed, La Jolla, CA 92093 USA

[2] Salk Inst Biol Studies, Clayton Fdn Labs Peptide Biol, La Jolla, CA 92037 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 270卷 / 02期

基金：

美国国家科学基金会;

关键词：

ascidian; celenamide; DOPA protein; halocyamine; mass spectrometry; oxidative decarboxylation; tunichrome;

D O I：

10.1006/bbrc.2000.2449

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plicatamide is a modified octapeptide from the ascidian Styela plicata having the structure Phe-Phe-His-Leu-His-Phe-His-decarboxy Delta DOPA (where decarboxy Delta DOPA = decarboxy- (E)-alpha,beta-dehydro-3,4-dihydroxyphenylalanine), Edman sequencing, tandem mass spectrometry, and proton NMR have been used to characterize 100 mu g of the compound. Plicatamide represents an important link between two classes of biomolecules: the tunichromes which share an oxidatively decarboxylated C-terminus and higher molecular weight DOPA-polypeptides. The 8-residue sequence provides the first opportunity to investigate the biosynthetic origins of the tunichrome family by molecular biological techniques. (C) 2000 Academic Press.

引用

页码：421 / 424

页数：4

共 16 条

[11] CELENAMIDE-A AND CELENAMIDE-B, LINEAR PEPTIDE ALKALOIDS FROM THE SPONGE CLIONA-CELATA [J].