Identification of sequence changes responsible for the attenuation of avian infectious bronchitis virus strain Arkansas DPI

被引:45
作者
Ammayappan, Arun [1 ,2 ]
Upadhyay, Chitra [1 ]
Gelb, Jack, Jr. [3 ]
Vakharia, Vikram N. [1 ]
机构
[1] Univ Maryland, Ctr Marine Biotechnol, Inst Biotechnol, Baltimore, MD 21202 USA
[2] Univ Maryland, Dept Vet Med, College Pk, MD 20742 USA
[3] Univ Delaware, Dept Anim & Food Sci, Coll Agr & Nat Resources, Newark, DE 19716 USA
基金
美国农业部;
关键词
ARTERIVIRUS REPLICATION COMPLEX; SPIKE GLYCOPROTEIN; MEMBRANE ASSOCIATION; CORONAVIRUS IBV; AMINO-ACIDS; S1; GENE; RECOMBINATION; PROTEIN; GENOME; POLYPROTEIN;
D O I
10.1007/s00705-009-0325-9
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Infectious bronchitis virus (IBV) is the causal agent of infectious bronchitis, which still remains one of the most important poultry diseases worldwide because of numerous serotypes and variants. A virulent strain of IBV, isolated from Arkansas (Ark), was propagated in embryonated eggs (Ark DPI 11). Following 101 serial passages in embryonated eggs, an attenuated strain of IBV was established (Ark DPI 101) that does not induce histopathological lesions in the tracheae of infected chicks. To identify sequence changes responsible for the attenuation of IBV, complete genome sequences of both virulent and attenuated Ark DPI viruses were obtained. Comparison of the genome sequences of the virulent and attenuated Ark DPI viruses reveals that these viruses are similar and differ only by 21 nucleotides, resulting in 17 amino acids changes. Most of those substitutions are located in the replicase 1a and spike genes. No differences were observed in gene 3, M or 5a, and only one nucleotide substitution each was present in 5b, N and 3'UTR. By comparing the deduced amino acid sequences of virulent and attenuated viruses, we identified sequence changes responsible for the adaptation and attenuation of the IBV-Ark DPI strain.
引用
收藏
页码:495 / 499
页数:5
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