A magnesium ion core at the heart of a ribozyme domain

被引：269

作者：

Cate, JH ^{[1
]}

Hanna, RL ^{[1
]}

Doudna, JA ^{[1
]}

机构：

[1] YALE UNIV, DEPT MOL BIOPHYS & BIOCHEM, NEW HAVEN, CT 06520 USA

来源：

NATURE STRUCTURAL BIOLOGY | 1997年 / 4卷 / 07期

关键词：

D O I：

10.1038/nsb0797-553

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Large ribozymes require divalent metal ions to fold. We show here that the tertiary structure of the Tetrahymena group I intron P4-P6 domain nucleates around a magnesium ion core. In the domain crystal structure, five magnesium ions bind in a three-helix junction at the centre of the molecule. Single atom changes in any one of four magnesium sites in this three-helix junction destroy folding of the entire 160-nucleotide P4-P6 domain. The magnesium ion core may be the RNA counterpart to the protein hydrophobic core, burying parts of the RNA molecule in the native structure.

引用

页码：553 / 558

页数：6

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