A magnesium ion core at the heart of a ribozyme domain

被引：269

作者：

Cate, JH ^{[1
]}

Hanna, RL ^{[1
]}

Doudna, JA ^{[1
]}

机构：

[1] YALE UNIV, DEPT MOL BIOPHYS & BIOCHEM, NEW HAVEN, CT 06520 USA

来源：

NATURE STRUCTURAL BIOLOGY | 1997年 / 4卷 / 07期

关键词：

D O I：

10.1038/nsb0797-553

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Large ribozymes require divalent metal ions to fold. We show here that the tertiary structure of the Tetrahymena group I intron P4-P6 domain nucleates around a magnesium ion core. In the domain crystal structure, five magnesium ions bind in a three-helix junction at the centre of the molecule. Single atom changes in any one of four magnesium sites in this three-helix junction destroy folding of the entire 160-nucleotide P4-P6 domain. The magnesium ion core may be the RNA counterpart to the protein hydrophobic core, burying parts of the RNA molecule in the native structure.

引用

页码：553 / 558

页数：6

共 42 条

[11] ZINC PROTEINS - ENZYMES, STORAGE PROTEINS, TRANSCRIPTION FACTORS, AND REPLICATION PROTEINS [J].

COLEMAN, JE .

ANNUAL REVIEW OF BIOCHEMISTRY, 1992, 61 :897-946

[12] DOMINANT FORCES IN PROTEIN FOLDING [J].

DILL, KA .

BIOCHEMISTRY, 1990, 29 (31) :7133-7155

[13] The P4-P6 domain directs higher order folding of the Tetrahymena ribozyme core [J].

Doherty, EA ;

Doudna, JA .

BIOCHEMISTRY, 1997, 36 (11) :3159-3169

[14] CRYSTALLIZATION OF RIBOZYMES AND SMALL RNA MOTIFS BY A SPARSE-MATRIX APPROACH [J].

DOUDNA, JA ;

GROSSHANS, C ;

GOODING, A ;

KUNDROT, CE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (16) :7829-7833

[15]

Downs WD, 1996, RNA, V2, P718

[16] BOND ORDER AND CHARGE LOCALIZATION IN NUCLEOSIDE PHOSPHOROTHIOATES [J].

FREY, PA ;

SAMMONS, RD .

SCIENCE, 1985, 228 (4699) :541-545

[17] DNA AND RNA SEQUENCE DETERMINATION BASED ON PHOSPHOROTHIOATE CHEMISTRY [J].

GISH, G ;

ECKSTEIN, F .

SCIENCE, 1988, 240 (4858) :1520-1522

[18] Protein stabilization by removal of unsatisfied polar groups: Computational approaches and experimental tests [J].

Hendsch, ZS ;

Jonsson, T ;

Sauer, RT ;

Tidor, B .

BIOCHEMISTRY, 1996, 35 (24) :7621-7625

[19]

HENDSCH ZS, 1994, PROTEIN SCI, V3, P211

[20] ABSOLUTE-CONFIGURATION OF RP-URIDINE 3',5'-CYCLIC PHOSPHOROTHIOATE [J].

HINRICHS, W ;

STEIFA, M ;

SAENGER, W ;

ECKSTEIN, F .

NUCLEIC ACIDS RESEARCH, 1987, 15 (12) :4945-4955

← 1 2 3 4 5 →