A novel in vitro cultivation system to produce and isolate soluble factors released from hyphae of arbuscular mycorrhizal fungi

被引：12

作者：

Gadkar, Vijay ^{[1
]}

Driver, James D. ^{[1
]}

Rillig, Matthias C. ^{[1
]}

机构：

[1] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA

来源：

BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 14期

关键词：

arbuscular mycorrhizal fungi; extramatrical; glomalin; hairy roots; root organ culture; symbiosis;

D O I：

10.1007/s10529-006-9055-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Petri plate dual in vitro culture system for arbuscular mycorrhizal fungi (AMF) is ill-suited for the production and isolation of extraradically released soluble factors, thereby preventing the further characterization of such compounds. To overcome this technical bottleneck, we here describe a novel cultivation system using the standard AMF Glomus intraradices-carrot dual culture as a model. This new system is capable of producing soluble factors in copious amounts without any interference from host roots. The greatest advantages of this culture method include ease of handling and reusability of the culture vessel, thus making it a very cost effective system.

引用

收藏

页码：1071 / 1076

页数：6

相关论文

共 14 条

[1] Nitrate depletion and pH changes induced by the extraradical mycelium of the arbuscular mycorrhizal fungus Glomus intraradices grown in monoxenic culture [J].

Bago, B ;

Vierheilig, H ;

Piche, Y ;

AzconAguilar, C .

NEW PHYTOLOGIST, 1996, 133 (02) :273-280

[2] ESTABLISHMENT OF VESICULAR-ARBUSCULAR MYCORRHIZA IN ROOT ORGAN-CULTURE - REVIEW AND PROPOSED METHODOLOGY [J].

BECARD, G ;

PICHE, Y .

METHODS IN MICROBIOLOGY, 1992, 24 :89-108

[3] SOLUBILIZATION OF GELLAN GELS BY CHELATION OF CATIONS [J].

DONER, LW ;

BECARD, G .

BIOTECHNOLOGY TECHNIQUES, 1991, 5 (01) :25-28

[4] Increased spore production by Glomus intraradices in the split-plate monoxenic culture system by repeated harvest, gel replacement, and resupply of glucose to the mycorrhiza [J].

Douds, DD .

MYCORRHIZA, 2002, 12 (04) :163-167

[5] Direct interaction between the arbuscular mycorrhizal fungus Glomus intraradices and different rhizosphere microorganisms [J].

Filion, M ;

St-Arnaud, M ;

Fortin, JA .

NEW PHYTOLOGIST, 1999, 141 (03) :525-533

[6] Arbuscular mycorrhizal phosphate transport under monoxenic conditions using radio-labelled inorganic and organic phosphate [J].

Joner, EJ ;

Ravnskov, S ;

Jakobsen, I .

BIOTECHNOLOGY LETTERS, 2000, 22 (21) :1705-1708

[7] A diffusible factor from arbuscular mycorrhizal fungi induces symbiosis-specific MtENOD11 expression in roots of Medicago truncatula [J].

Kosuta, S ;

Chabaud, M ;

Lougnon, G ;

Gough, C ;

Dénarié, J ;

Barker, DG ;

Bécard, G .

PLANT PHYSIOLOGY, 2003, 131 (03) :952-962

[8] Nod factors and a diffusible factor from arbuscular mycorrhizal fungi stimulate lateral root formation in Medicago truncatula via the DMI1/DMI2 signalling pathway [J].

Oláh, B ;

Brière, C ;

Bécard, G ;

Dénarié, J ;

Gough, C .

PLANT JOURNAL, 2005, 44 (02) :195-207

[9] Glomalin production by an arbuscular mycorrhizal fungus: a mechanism of habitat modification? [J].

Rillig, MC ;

Steinberg, PD .

SOIL BIOLOGY & BIOCHEMISTRY, 2002, 34 (09) :1371-1374

[10] Arbuscular mycorrhizae and terrestrial ecosystem processes [J].

Rillig, MC .

ECOLOGY LETTERS, 2004, 7 (08) :740-754