Regulation of period 1 expression in cultured rat pineal

被引：31

作者：

Fukuhara, C ^{[1
]}

Dirden, JC ^{[1
]}

Tosini, G ^{[1
]}

机构：

[1] Morehouse Sch Med, Inst Neurosci, Atlanta, GA 30310 USA

来源：

NEUROSIGNALS | 2002年 / 11卷 / 02期

关键词：

period; 1; arylalkylamine N-acetyltransferase; cAMP; cGMP; MAPK; e-box; circadian;

D O I：

10.1159/000058547

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of the present study was to investigate the in vitro expression of Period 1 (Per1), Period 2 (Per2) and arylalkylamine N-acetyltransferase (AA-NAT) genes in the rat pineal gland to understand the mechanism(s) regulating the expression of these genes in this organ. Pineals, when maintained in vitro for 5 days, did not show circadian rhythmicity in the expression of any of the three genes monitored. Norepinephrine (NE) induced AA-NAT and Per1, whereas its effect on Per2 was negligible. Contrary to what was observed in other systems, NE stimulation did not induce circadian expression of Per1. The effect of NE on Per1 level was dose- and receptor subtype-dependent, and both cAMP and cGMP induced Per1. Per1 was not induced by repeated NE - or forskolin - stimulation. Protein synthesis was not necessary for NE-induced Per1, but it was for reduction of Per1 following NE stimulation. Per1 transcription in pinealo-cytes was activated by BMAL1/CLOCK. Our results indicate that important differences are present in the regulation of these genes in the mammalian pineal.

引用

页码：103 / 114

页数：12

共 34 条

[1]

Akashi M, 2000, GENE DEV, V14, P645

[2] PD-098059 IS A SPECIFIC INHIBITOR OF THE ACTIVATION OF MITOGEN-ACTIVATED PROTEIN-KINASE KINASE IN-VITRO AND IN-VIVO [J].

ALESSI, DR ;

CUENDA, A ;

COHEN, P ;

DUDLEY, DT ;

SALTIEL, AR .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (46) :27489-27494

[3] Rat arylalkylamine N-acetyltransferase gene:: Upstream and intronic components of a bipartite promoter [J].

Baler, R ;

Covington, S ;

Klein, DC .

BIOLOGY OF THE CELL, 1999, 91 (09) :699-705

[4] Multiple signaling pathways elicit circadian gene expression in cultured Rat-1 fibroblasts [J].

Balsalobre, A ;

Marcacci, L ;

Schibler, U .

CURRENT BIOLOGY, 2000, 10 (20) :1291-1294

[5] Resetting of circadian time peripheral tissues by glucocorticoid signaling [J].

Balsalobre, A ;

Brown, SA ;

Marcacci, L ;

Tronche, F ;

Kellendonk, C ;

Reichardt, HM ;

Schütz, G ;

Schibler, U .

SCIENCE, 2000, 289 (5488) :2344-2347

[6] A serum shock induces circadian gene expression in mammalian tissue culture cells [J].

Balsalobre, A ;

Damiola, F ;

Schibler, U .

CELL, 1998, 93 (06) :929-937

[7] The rat arylalkylamine N-acetyltransferase E-box:: differential use in a master vs. a slave oscillator [J].

Chen, W ;

Baler, R .

MOLECULAR BRAIN RESEARCH, 2000, 81 (1-2) :43-50

[8] SUPERSENSITIVITY AND SUBSENSITIVITY OF BETA-ADRENERGIC RECEPTOR IN PINEAL-GLAND REGULATED BY CATECHOLAMINE TRANSMITTER [J].

DEGUCHI, T ;

AXELROD, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (08) :2411-2414

[9] Circadian expression of Period 1, Period 2, and arylalkylamine N-acetyltransferase rnRNA in the rat pineal gland under different light conditions [J].

Fukuhara, C ;

Dirden, JC ;

Tosini, G .

NEUROSCIENCE LETTERS, 2000, 286 (03) :167-170

[10] The human and mouse Period1 genes:: Five well-conserved E-boxes additively contribute to the enhancement of mPer1 transcription [J].

Hida, A ;

Koike, N ;

Hirose, M ;

Hattori, M ;

Sakaki, Y ;

Tei, H .

GENOMICS, 2000, 65 (03) :224-233

← 1 2 3 4 →