Interferon-γ and interleukin-4 downregulate expression of the SARS coronavirus receptor ACE2 in Vero E6 cells

被引：103

作者：

de Lang, Anna ^{[1
]}

Osterhaus, Albert D. M. E. ^{[1
]}

Haagmans, Bart L. ^{[1
]}

机构：

[1] Erasmus MC, Dept Virol, NL-3015 GE Rotterdam, Netherlands

来源：

VIROLOGY | 2006年 / 353卷 / 02期

关键词：

SARS-CoV; ACE2; interferon-gamma; interleukin-4;

D O I：

10.1016/j.virol.2006.06.011

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Interferons (IFNs) inhibit severe acute respiratory syndrome coronavirus (SARS-CoV) replication and might be valuable for SARS treatment. In this study, we demonstrate that treatment of Vero E6 cells with interleukin-4 (IL-4) decreased the susceptibility of these cells to SARS-CoV infection. In contrast to IFNs, IL-4 did not show antiviral activity when administered immediately after SARS-CoV infection, suggesting that IL-4 acts early during the SARS-CoV replication cycle. Indeed, binding of recombinant SARS-CoV spike protein to Vero E6 cells was diminished on cells treated with IL-4, but also on cells exposed to IFN-gamma. Consistent with these observations, IL-4 and IFN-gamma downregulated cell surface expression of angiotensin-converting enzyme 2 (ACE2), the SARS-CoV receptor. Besides diminished ACE2 cell surface expression, ACE2 mRNA levels were also decreased after treatment with these cytokines. These findings suggest that IL-4 and IFN-gamma inhibit SARS-CoV` replication partly through downregulation of ACE2. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：474 / 481

页数：8

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