Catalytic self-screening of cholinesterase substrates from a dynamic combinatorial thioester library

被引：79

作者：

Larsson, R ^{[1
]}

Pei, ZC ^{[1
]}

Ramström, O ^{[1
]}

机构：

[1] Royal Inst Technol, Dept Chem, S-10044 Stockholm, Sweden

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 28期

关键词：

combinatorial chemistry; enzyme catalysis hydrolysis; proteomics; transesterification;

D O I：

10.1002/anie.200454165

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Simply the best: Dynamic combinatorial chemistry coupled to enzyme catalysis was used to identify enzyme substrates in a library constructed from a series of thioesters and thiols by transesterification. The library was directly coupled to the catalytic action of acetylcholinesterase, which selectively hydrolyzed the best substrate candidates (see schematic representation). The process allowed rapid identification of discrete substrates.

引用

页码：3716 / 3718

页数：3

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