Unnatural base pair systems for DNA/RNA-based biotechnology

被引：126

作者：

Hirao, Ichiro ^{[1
]}

机构：

[1] RIKEN, Genom Sci Ctr, Prot Res Grp, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2006年 / 10卷 / 06期

关键词：

D O I：

10.1016/j.cbpa.2006.09.021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of unnatural, extra base pairs could expand genetic information, by enabling the site-specific incorporation of functional components into nucleic acids and proteins. Researchers have been trying to create unnatural base pairs by drawing upon non-standard hydrogen-bonding topologies, shape complementarity, and/or hydrophobic interfaces, to confer sufficient selectivity and efficiency to work with the natural A-T(U) and G-C base pairs in replication, transcription and translation. The development of artificial genetic systems has been broadly advanced by combining the extra base pairs with modified natural components, including the base pairs, substrates and polymerase enzymes.

引用

页码：622 / 627

页数：6

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