Properties of a glucosyltransferase involved in crocin synthesis

被引:8
作者
Côté, F
Cormier, F
Dufresne, C
Willemot, C
机构
[1] Agr & Agri Food Canada, Ctr Food Res & Dev, St Hyacinthe, PQ J2S 8E3, Canada
[2] Univ Laval, Dept Plant Sci, Hort Res Ctr, St Foy, PQ G1K 7P4, Canada
关键词
saffron; Crocus sativus; glucosyltransferase; crocetin; glycosylation; carotenoids;
D O I
10.1016/S0168-9452(99)00248-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The properties of a partially purified uridine-5'-diphosphoglucose (UDP-glucose)- crocetin 8,8'-glucosyltransferase (EC 2.4.1.) involved in crocin synthesis are described. The enzyme participates in the biosynthesis of crocin by forming ester bonds between the carboxyl groups of crocetin and the glucose moiety of UDP-glucose. The enzyme has been purified 125-fold trough a multi-step process of acetone fractionation, anion-exchange, and gel filtration chromatography. The native molecular weight is in the range of 47 kDa, as estimated by analytical gel filtration. The most purified preparation showed isoforms between pH 4.4 and 4.8 when resolved by IEF. The enzyme had maximal activity at 40 degrees C, apparent K-m values for UDP-glucose and crocetin were 0.72 and 0.17 mM, respectively, and V-max, 30 pkatal/mg. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:55 / 63
页数:9
相关论文
共 23 条
[1]  
BERGMEYER HU, 1984, METHOD ENZYMAT AN, V4, P241
[2]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3]   ENHANCED CROCETIN GLUCOSYLATION BY MEANS OF MALTOSYL-BETA-CYCLODEXTRIN ENCAPSULATION [J].
CORMIER, F ;
DUFRESNE, C ;
DORION, S .
BIOTECHNOLOGY TECHNIQUES, 1995, 9 (08) :553-556
[4]  
CORNISHBOWDEN A, 1995, FUNDAMENTALS ENZYME, P55
[5]  
DEKKER RFH, 1981, J GEN MICROBIOL, V127, P177
[6]   In vitro formation of crocetin glucosyl esters by Crocus sativus callus extract [J].
Dufresne, C ;
Cormier, F ;
Dorion, S .
PLANTA MEDICA, 1997, 63 (02) :150-153
[7]   Glycosylation of encapsulated crocetin by a Crocus sativus L-cell culture [J].
Dufresne, C ;
Cormier, F ;
Dorion, S ;
Niggli, UA ;
Pfister, S ;
Pfander, H .
ENZYME AND MICROBIAL TECHNOLOGY, 1999, 24 (8-9) :453-462
[8]  
Hosel W., 1981, The biochemistry of plants. A comprehensive treatise. Volume 7. Secondary plant products., P725
[9]   PURIFICATION AND PARTIAL CHARACTERIZATION OF UDP-GLUCOSE - PHENOL-BETA-D-GLUCOSYLTRANSFERASE FROM PAPAYA FRUIT [J].
KEIL, U ;
SCHREIER, P .
PHYTOCHEMISTRY, 1989, 28 (09) :2281-2284
[10]   Regulation of triacylglucose fatty acid composition - Uridine diphosphate glucose fatty acid glucosyltransferases with overlapping chain-length specificity [J].
Kuai, JP ;
Ghangas, GS ;
Steffens, JC .
PLANT PHYSIOLOGY, 1997, 115 (04) :1581-1587