A Tubular Biocontainer: Metal Ion-Induced 1D Assembly of a Molecularly Engineered Chaperonin

被引：81

作者：

Biswas, Shuvendu ^{[1
,2
]}

Kinbara, Kazushi ^{[1
,2
]}

Oya, Nobuhiro ^{[1
,2
]}

Ishii, Noriyuki ^{[3
]}

Taguchi, Hideki ^{[4
]}

Aida, Takuzo ^{[1
,2
]}

机构：

[1] Univ Tokyo, Dept Chem & Biotechnol, Sch Engn, Bunkyo Ku, Tokyo 1138656, Japan

[2] Univ Tokyo, Ctr NanoBio Integrat, Bunkyo Ku, Tokyo 1138656, Japan

[3] Natl Inst Adv Ind Sci & Technol, Biol Informat Res Ctr, Tsukuba, Ibaraki 3058566, Japan

[4] Univ Tokyo, Dept Med Genome Sci, Grad Sch Frontier Sci, Kashiwa, Chiba 2778562, Japan

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 22期

关键词：

FILAMENTS; SHAPE;

D O I：

10.1021/ja902696q

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

GroEL(SP/MC), prepared by genetic and chemical modifications of group I chaperonin protein GroEL, site-specifically possesses similar to 28 photochromic (spiropyran [SP] and merocyanine [MC]) units in the entrance parts of its cavity. Addition of divalent metal ions such as Mg2+ to a tris-HCl buffer solution of GroEL(SP/MC) results in one-dimensional (1D) assembly of GroEL(SP/MC), affording cylindrical hollow fibers with a very large aspect ratio; the longest fiber was similar to 2.5 mu m tong, corresponding to a 170-mer of GroEL(SP/MC) (MW approximate to 1.4 x 10(8)). When such tong fibers are mixed with EDTA, they are cut into short-chain oligomers and eventually into monomeric GroEL(SP/MC). Similar to GroEL, GroEL(SP/MC) possesses a large binding affinity toward denatured proteins. When GroEL(SP/MC) undergoes 1D assembly after incubation with a denatured protein, guest-containing cylindrical fibers result.

引用

页码：7556 / +

页数：4

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