Taxonomic position and intraspecific variability of the nodule forming Penicillium nodositatum inferred from RFLP analysis of the ribosomal intergenic spacer and random amplified polymorphic DNA

被引:53
作者
Sequerra, J
Marmeisse, R
Valla, G
Normand, P
Capellano, A
Moiroud, A
机构
[1] Univ.́ Claude Bernard Lyon 1, Ecologie Microbienne du Sol UMR C., Bât. 405, F 69622 Villeurbanne Cedex, 43
来源
MYCOLOGICAL RESEARCH | 1997年 / 101卷
关键词
D O I
10.1017/S0953756296002857
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Penicillium nodositatum induces the formation of nodules on the roots of Alnus. The analysis of RFLPs in the PCR-amplified ITS1-5.8S-ITS2 ribosomal sequence demonstrates that all the nodule-inducing strains isolated belong to a homogeneous taxon, P. nodositatum, distinct from other morphologically close species of Penicillium. A gene tree inferred from the ITS1-5.8S-ITS2 PCR/RFLP data shows that the P, nodositatum sequence clusters with those of Penicillium species belonging to subgenus Biverticillium. RAPDs generated using four 10-mer primers, allowed us to identify four distinct groups of strains in a sample of 28 strains of P. nodositatum collected in 10 different geographic sites. Only one group was found in each sampled site. No link could be established between RAPD-based groups and the geographic origin of the strains. However, our results suggest a possible link between these groups and Alnus species and/or habitats associated with each of the four French Alnus species.
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收藏
页码:465 / 472
页数:8
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