Not lipoteichoic acid but lipoproteins appear to be the dominant immunobiologically active compounds in Staphylococcus aureus

被引:186
作者
Hashimoto, Masahito
Tawaratsumida, Kazuki
Kariya, Hiroyuki
Kiyohara, Ai
Suda, Yasuo
Krikae, Fumiko
Kirikae, Teruo
Goetz, Friedrich
机构
[1] Kagoshima Univ, Dept Nanostruct & Adv Mat, Kagoshima 8900065, Japan
[2] Int Med Ctr Japan, Dept Infect Dis, Tokyo, Japan
[3] Univ Tubingen, Tubingen, Germany
关键词
D O I
10.4049/jimmunol.177.5.3162
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Lipoteichoic acid (LTA) derived from Staphylococcus aureus is reported to be a ligand of TLR2. However, we previously demonstrated that LTA fraction prepared from bacterial cells contains lipoproteins, which activate cells via TLR2. In this study, we investigated the immunobiological activity of LTA fraction obtained from S. aureus wild-type strain, lipoprotein diacylglycerol transferase deletion (Delta lgt) mutant, which lacks palmitate-labeled lipoproteins, and its complemented strain and evaluated the activity of LTA molecule. LTA fraction was prepared by butanol extraction of the bacteria followed by hydrophobic interaction chromatography. Although all LTA fractions activated cells through TLR2, the LTA from Delta lgt mutant was 100-fold less potent than those of wild-type and complemented strains. However, no significant structural difference in LTA was observed in NMR spectra. Further, alanylation of LTA molecule showed no effect in immunobiological activity. These results showed that not LTA molecule but lipoproteins are dominant immunobiologically active TLR2 ligand in S. aureus.
引用
收藏
页码:3162 / 3169
页数:8
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