Bipolar membrane electroacidification to produce bovine milk casein isolate

被引:55
作者
Bazinet, L
Lamarche, F
Ippersiel, D
Amiot, J
机构
[1] Agr & Agri Food Canada, Ctr Food Res & Dev, St Hyacinthe, PQ J2S 8E3, Canada
[2] Univ Laval, Ctr Rech Sci & Technol Lait, Ste Foy, PQ G1K 7P4, Canada
关键词
electrochemical acidification; bipolar membrane; casein; milk; precipitation;
D O I
10.1021/jf990524m
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Bipolar membrane electroacidification (BM:EA) has been developed previously (Bazinet et al., Report for the Canadian Electricity Association 9326 U 987, 1996; Bazinet et al., J. Agric. Food Chem. 1997, 45, 2419-2425, 3788-3794) and has been used for isoelectric precipitation of soybean proteins. The purpose of this study was to validate the feasibility of BMEA for the precipitation of milk casein and to investigate the effect of flow rate. High-purity isolates containing 1.23 and 2.00% ash and 85.4 and 91.6% total protein were obtained with flow rates of 0.2 and 1.2 gal/min. The molecular composition profiles of the isolates obtained by HPLC showed that only caseins were precipitated. However, except for protein precipitation curves, the flow rate did not influence the final composition and purity of the isolates. These results showed that BMEA is a new alternative process for the production of high-purity bovine milk casein isolate.
引用
收藏
页码:5291 / 5296
页数:6
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