The luminal part of the murine cytomegalovirus glycoprotein gp40 catalyzes the retention of MHC class I molecules

被引:71
作者
Ziegler, H
Muranyi, W
Burgert, HG
Kremmer, E
Koszinowski, UH
机构
[1] Univ Munich, Max Von Pettenkofer Inst, Lehrstuhl Virol, Genzentrum, D-80336 Munich, Germany
[2] GSF Forschungszentrum Umwelt & Gesundheit, Inst Mol Immunol, D-81377 Munich, Germany
关键词
antigen presentation; ERGIC; cis-Golgi retention; immune evasion; MHC class I pathway; murine cytomegalovirus;
D O I
10.1093/emboj/19.5.870
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Murine cytomegalovirus (MCMV) interferes with the MHC class I pathway of antigen presentation. The type I transmembrane glycoprotein gp40, encoded by the gene m152, retains major histocompatibility complex (MHC) class I complexes in the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC)/cis-Golgi. These MHC class I complexes are stable, show an extended half-life and do not exchange Pz-microglobulin, whereas gp40 reaches an endosomal/ lysosomal compartment where it subsequently is degraded. The analysis of regions within the viral protein that are essential for MHC class I retention revealed that a secreted form of gp40, lacking the cytoplasmic tail and the transmembrane region, still has the capacity to retain MHC class I complexes, Continuous expression of gp40 is not required for MHC class I retention, Our data indicate that the retention of MHC class I complexes in the ERGIC/ cis-Golgi is triggered by gp40 and does not require the further presence of the viral protein.
引用
收藏
页码:870 / 881
页数:12
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