Enhancement of lipid production using biochemical, genetic and transcription factor engineering approaches

被引:367
作者
Courchesne, Noemie Manuelle Dorval [1 ]
Parisien, Albert [1 ]
Wang, Bei [1 ]
Lan, Christopher Q. [1 ]
机构
[1] Univ Ottawa, Dept Chem & Biol Engn, Ottawa, ON K1N 6N5, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
Biodiesel; Biofuel; Microalgae; Lipid; Metabolic engineering; Transcription factor; ACETYL-COA CARBOXYLASE; FATTY-ACID SYNTHESIS; FACTOR-BINDING SITES; SEED OIL CONTENT; ESCHERICHIA-COLI; CANDIDA-TROPICALIS; TRANSGENIC PLANTS; CALORIFIC VALUES; MALIC ENZYME; COENZYME-A;
D O I
10.1016/j.jbiotec.2009.02.018
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This paper compares three possible strategies for enhanced lipid overproduction in microalgae: the biochemical engineering (BE) approaches, the genetic engineering (GE) approaches, and the transcription factor engineering (TFE) approaches. The BE strategy relies on creating a physiological stress such as nutrient-starvation or high salinity to channel metabolic fluxes to lipid accumulation. The GE strategy exploits Our understanding to the lipid metabolic pathway, especially the rate-limiting enzymes, to create a channelling of metabolites to lipid biosynthesis by overexpressing one or more key enzymes in recombinant microalgal strains. The TFE strategy is an emerging technology aiming at enhancing the production of a particular metabolite by means of overexpressing TFs regulating the metabolic pathways involved in the accumulation of target metabolites. Currently, BE approaches are the most established in microalgal lipid production. The TFE is a very promising strategy because it may avoid the inhibitive effects of the BE approaches and the limitation of "secondary bottlenecks" as commonly observed in the GE approaches. However, it is still a novel concept to be investigated systematically. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:31 / 41
页数:11
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