Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization

被引：276

作者：

Roux, Kyle J. ^{[1
]}

Crisp, Melissa L. ^{[1
]}

Liu, Qian ^{[1
]}

Kim, Daein ^{[1
]}

Kozlov, Serguei ^{[3
,4
]}

Stewart, Colin L. ^{[2
]}

Burke, Brian ^{[1
]}

机构：

[1] Univ Florida, Dept Anat & Cell Biol, Gainesville, FL 32610 USA

[2] 8A Biomed Grove, Med Biol Inst, Immunos, Singapore 138648, Singapore

[3] SAIC Frederick Inc, Natl Canc Inst, Ctr Adv Preclin Res, Ft Detrick, MD 21702 USA

[4] SAIC Frederick Inc, Natl Canc Inst, Mouse Canc Genet Program, Ft Detrick, MD 21702 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2009年 / 106卷 / 07期

基金：

美国国家卫生研究院;

关键词：

centrosome; LINC complex; nuclear envelope; SPECTRIN SUPERFAMILY; DOMAIN PROTEINS; LAMIN-A; ENVELOPE; MIGRATION; LOCALIZATION; MICROTUBULES; CYTOSKELETON; EXPRESSION; DROSOPHILA;

D O I：

10.1073/pnas.0808602106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nucleocytoplasmic coupling is mediated by outer nuclear membrane (ONM) nesprin proteins and inner nuclear membrane Sun proteins. Interactions spanning the perinuclear space create nesprin-Sun complexes connecting the cytoskeleton to nuclear components. A search for proteins displaying a conserved C-terminal sequence present in nesprins 1-3 identified nesprin 4 (Nesp4), a new member of this family. Nesp4 is a kinesin-1-binding protein that displays Sun-dependent localization to the ONM. Expression of Nesp4 is associated with dramatic changes in cellular organization involving relocation of the centrosome and Golgi apparatus relative to the nucleus. These effects can be accounted for entirely by Nesp4's kinesin-binding function. The implication is that Nesp4 may contribute to microtubule-dependent nuclear positioning.

引用

页码：2194 / 2199

页数：6

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