The promise of structural genomics in the discovery of new antimicrobial agents

被引:11
作者
Buchanan, SG [1 ]
Sauder, JM [1 ]
Harris, T [1 ]
机构
[1] Struct GenomiX, Bioinformat, San Diego, CA 92121 USA
关键词
D O I
10.2174/1381612023394809
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Structural Genomics stands out among the emerging fields of proteomics since it influences the drug discovery process at so many points. Recent developments in protein expression technologies, x-ray crystallography and NMR spectroscopy provide the essential elements for high-throughput structure determination platforms. Bioinformatics methods to interrogate the resulting data will provide comprehensive, genome-wide databases of protein structure. Genomic sequencing and methods for high-throughput expression and protein purification are furthest advanced for microbial genes and so these have been the early targets for structural genomics initiatives. The information will be invaluable in understanding gene function, designing broad-spectrum small molecule inhibitors and in better understanding drug-host interactions.
引用
收藏
页码:1173 / 1188
页数:16
相关论文
共 120 条
[41]   A systematic comparison of protein structure classifications: SCOP, CATH and FSSP [J].
Hadley, C ;
Jones, DT .
STRUCTURE WITH FOLDING & DESIGN, 1999, 7 (09) :1099-1112
[42]   Identification of novel inhibitors of urokinase via NMR-based screening [J].
Hajduk, PJ ;
Boyd, S ;
Nettesheim, D ;
Nienaber, V ;
Severin, J ;
Smith, R ;
Davidson, D ;
Rockway, T ;
Fesik, SW .
JOURNAL OF MEDICINAL CHEMISTRY, 2000, 43 (21) :3862-3866
[43]  
HENDRICKSON WA, 1989, BASIC LIFE SCI, V51, P317
[44]   Synchrotron crystallography [J].
Hendrickson, WA .
TRENDS IN BIOCHEMICAL SCIENCES, 2000, 25 (12) :637-643
[45]   The structural basis of ordered substrate binding by serotonin N-acetyltransferase:: Enzyme complex at 1.8 Å resolution with a bisubstrate analog [J].
Hickman, AB ;
Namboodiri, MAA ;
Klein, DC ;
Dyda, F .
CELL, 1999, 97 (03) :361-369
[46]   Sliding clamps: A (tail)ored fit [J].
Hingorani, MM ;
O'Donnell, M .
CURRENT BIOLOGY, 2000, 10 (01) :R25-R29
[47]   The FSSP database: Fold classification based on structure structure alignment of proteins [J].
Holm, L ;
Sander, C .
NUCLEIC ACIDS RESEARCH, 1996, 24 (01) :206-209
[48]   The three-dimensional structure of capsule-specific CMP: 2-keto-3-deoxy-manno-octonic acid synthetase from Escherichia coli [J].
Jelakovic, S ;
Jann, K ;
Schulz, GE .
FEBS LETTERS, 1996, 391 (1-2) :157-161
[49]   Identification and characterization of a p53 homologue in Drosophila melanogaster [J].
Jin, SK ;
Martinek, S ;
Joo, WS ;
Wortman, JR ;
Mirkovic, N ;
Sali, A ;
Yandell, MD ;
Pavletich, NP ;
Young, MW ;
Levine, AJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (13) :7301-7306
[50]  
Jones DT, 1999, PROTEINS, P104