Protective effects of the total saponins from Rosa laevigata Michx fruit against carbon tetrachloride-induced acute liver injury in mice

被引：53

作者：

Dong, Deshi ^{[1
,2
,3
]}

Zhang, Shuai ^{[1
]}

Yin, Lianhong ^{[1
]}

Tang, Xinqiang ^{[3
]}

Xu, Youwei ^{[1
]}

Han, Xu ^{[1
]}

Qi, Yan ^{[1
]}

Peng, Jinyong ^{[1
,2
]}

机构：

[1] Dalian Med Univ, Coll Pharm, Dalian 116044, Peoples R China

[2] Dalian Med Univ, Res Inst Integrated Tradit & Western Med, Dalian 116011, Peoples R China

[3] Dalian Med Univ, Affiliated Hosp 1, Dalian 116011, Peoples R China

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2013年 / 62卷

关键词：

Acute liver injury; Carbon tetrachloride; Hepatoprotective effect; Rosa laevigata Michx; Total saponins; ENDOPLASMIC-RETICULUM STRESS; INDUCED OXIDATIVE DAMAGE; NF-KAPPA-B; HEPATOPROTECTIVE ACTIVITY; INDUCED APOPTOSIS; TOTAL FLAVONOIDS; ER STRESS; IN-VITRO; RATS; HEPATOTOXICITY;

D O I：

10.1016/j.fct.2013.08.050

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

Saponins are the major chemicals from Rosa laevigata Michx fruit. However, there have no papers to report the activities of the natural product against liver damage. In the present work, total saponins from R. laevigata Michx fruit (RLTS) with purity >70% was produced and then the protective effects of it against CCl4-induced acute liver injury in mice was tested. The results showed that RLTS decreased serum ALT and AST activities compared with model group, as well as the relative liver weight and histological findings. In addition, RLTS remarkably increased the levels of SOD, CAT, GSH-Px, GSH and decreased MDA, iNOS and NO levels in liver. Transmission electron microscopy and TUNEL assays showed that RLTS repaired fragmented DNA and mitochondrial change caused by CCl4. Further investigation demonstrated that RLTS pretreatment down-regulated the protein expression of CYP2E1, ATF6, GRP78, EIF2, COX-2, NF-kappa B, p53, Caspase-3, Caspase-9, Cytokeratin 18 and the levels of MAPKs phosphorylation, up-regulated Bcl-2 expression, and markedly decreased the gene expression of TNF-alpha, IL-6, Fas/FasL and Bax. This is the first time to reveal the hepatoprotective effect of total saponins from R. laevigata Michx fruit, which should be developed as a new drug for treatment of live injury in future. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：120 / 130

页数：11

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