Genotype characterisation of Giardia duodenalis isolates from domestic and farm animals by SSU-rRNA gene sequencing

被引:75
作者
Berrilli, F
Di Cave, D
De Liberato, C
Franco, A
Scaramozzino, P
Orecchia, P
机构
[1] Univ Roma Tor Vergata, Dipartimento Sanita Pubbl & Biol Cellulare, Cattedra Parassirol, I-00133 Rome, Italy
[2] Ist Zooprofilatt Sperimentale Reg Lazio & Toscana, I-00178 Rome, Italy
关键词
Giardia duodenalis; dog; cat; cattle; PCR; genotype;
D O I
10.1016/j.vetpar.2004.04.008
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
In order to investigate the genotypes of Giardia duodenalis from domestic and farm animals in Italy, 21 Giardia isolates, 17 from dogs, 1 from cat and 3 from dairy calves, were genetically characterised by SSU-rRNA gene sequencing. Among dogs, 76.5% of isolates showed the dog-specific genotypes (Assemblages C, D and C/D mixed Assemblage) and 23.5% exhibit potential zoonotic genotypes (Assemblage A and A/C mixed Assemblages). The cat isolate belonged to assemblage A, whereas the sequences among the isolates from calves were found to correspond to hoofed-livestock genotype, namely Assemblage E. These findings suggest that infection of humans by zoonotic genotypes from domestic animals could be of low epidemiological significance, although possible. The present study represents the first contribute to the knowledge of G. duodenalis genotypes in domestic and farm animals from Italy. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:193 / 199
页数:7
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