Microfluidic Generation of Lipidic Mesophases for Membrane Protein Crystallization

被引：39

作者：

Perry, Sarah L. ^{[1
]}

Roberts, Griffin W. ^{[1
]}

Tice, Joshua D. ^{[1
]}

Gennis, Robert B. ^{[2
]}

Kenis, Paul J. A. ^{[1
]}

机构：

[1] Univ Illinois, Dept Chem & Biomol Engn, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Biochem, Urbana, IL 61801 USA

来源：

CRYSTAL GROWTH & DESIGN | 2009年 / 9卷 / 06期

关键词：

CUBIC PHASES; FORMULATOR; MATRICES; DIAGRAM; MIXER;

D O I：

10.1021/cg900289d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report on a microfluidic method for the formation of aqueous/lipid mesophases to enable screening of suitable crystallization conditions of membrane proteins from a membrane-like phase in sub-20 nL volumes. This integrated microfluidic chip and the employed mixing strategy address the specific challenges associated with the mixing of fluids of highly different viscosities (here a factor of 30) as well as the non-Newtonian character of the resulting mesophases. The chip requires less than 20 nL of material per condition screened, whereas typically on the order of 10 mu L or more is needed for a batch preparation in the present screening methods. We validated our approach with the successful crystallization of the membrane protein bacteriorhodopsin.

引用

页码：2566 / 2569

页数：4