New wine from old barrels

被引：25

作者：

Gerlt, JA

机构：

[1] Univ Illinois, Dept Biochem, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

来源：

NATURE STRUCTURAL BIOLOGY | 2000年 / 7卷 / 03期

关键词：

D O I：

10.1038/73249

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphoribosylanthranilate isomerase (PRAI) and indole-3-glycerol phosphate synthase (IGPS) are distantly related α/β-barrel enzymes that catalyze successive steps in tryptophan biosynthesis. A ‘new’ PRAI was evolved from an ‘old’ IGPS by random mutagenesis followed by DNA shuffling and in vivo selection.

引用

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页码：171 / 173

页数：3

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