A novel immobilized cholinesterase for on-site screening of organophosphate and carbamate compounds

被引：17

作者：

Zou, Ming-Qiang ^{[1
]}

Yang, Rul

Wang, Da-Ning

Li, Jin-Feng

Jin, Qin-Han

机构：

[1] Chinese Acad Inspect & Quarantine, Beijing 100025, Peoples R China

[2] Jilin Univ, Coll Chem, Changchun 130023, Peoples R China

来源：

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY | 2006年 / 86卷 / 03期

关键词：

immobilized cholinesterase; polyvinylpyrrolidone K30 (PVP); on-site screening; organophosphate and carbamate compounds;

D O I：

10.1016/j.pestbp.2006.03.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acetylcholinesterase was physically adsorbed on polyvinylpyrrolidone K30 to prepare a novel immobilized cholinesterase, which was described for the first time. The immobilized enzyme was tested for its reactivity, enzyme activity stability, the solubility, the influence of pH and buffer, and characteristic reactivity with inhibitors, e.g., organophosphate and carbarnate compounds. The result revealed that the immobilized cholinesterase held its original activity in solution, stable enough, easy to dissolve for convenient operation, and this immobilized cholinesterase could be well used for rapid, on-site screening of organophosphate and carbarnate compounds, together with a portable pesticide analyzer. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：162 / 166

页数：5

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