Disulfide structure of the pheromone binding protein from the silkworm moth, Bombyx mori

被引：211

作者：

Leal, WS ^{[1
]}

Nikonova, L ^{[1
]}

Peng, GH ^{[1
]}

机构：

[1] Natl Inst Sericultural & Entomol Sci, Lab Chem Prospecting, Tsukuba, Ibaraki 3058634, Japan

来源：

FEBS LETTERS | 1999年 / 464卷 / 1-2期

关键词：

enzymatic digestion; disulfide linkage; ESI-MS; TCEP; carboxyamidomethyl cysteine; PBP;

D O I：

10.1016/S0014-5793(99)01683-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Disulfide bond formation is the only known posttranslational modification of insect pheromone binding proteins (PBPs), In the PBPs from moths (Lepidoptera), six cysteine residues are highly conserved at positions 19, 50, 54, 97, 108 and 117, but to date nothing is known about their respective linkage or redox status, We used a multiple approach of enzymatic digestion, chemical cleavage, partial reduction with Tris-(2-carboxyethyl)phosphine, followed by digestion with endoproteinase Lys-C to determine the disulfide connectivity in the PBP from Bombyx mori (BmPBP), Identification of the reaction products by on-line liquid chromatography-electrospray ionization mass spectrometry (LC/ESI-MS) and protein sequencing supported the assignment of disulfide bridges at Cys-19-Cys-54, Cys-50-Cys-108 and Cys-97-Cys-117. The disulfide linkages were identical in the protein obtained by periplasmic expression in Escherichia coli and in the native BmPBP. (C) 1999 Federation of European Biochemical Societies.

引用

页码：85 / 90

页数：6

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