Circulating MicroRNAs as Biomarkers of Acute Stroke

被引：150

作者：

Sepramaniam, Sugunavathi ^{[1
,2
]}

Tan, Jun-Rong ^{[1
,2
]}

Tan, Kay-Sin ^{[3
]}

DeSilva, Deidre Ann ^{[4
,5
]}

Tavintharan, Subramaniam ^{[6
,7
]}

Woon, Fung-Peng ^{[4
,5
]}

Wang, Chee-Woon ^{[8
]}

Yong, Fung-Lin ^{[3
]}

Karolina, Dwi-Setyowati ^{[1
,2
]}

Kaur, Prameet ^{[1
,2
]}

Liu, Fu-Jia ^{[1
,2
]}

Lim, Kai-Ying ^{[1
,2
]}

Armugam, Arunmozhiarasi ^{[1
,2
]}

Jeyaseelan, Kandiah ^{[1
,2
,9
]}

机构：

[1] Natl Univ Singapore, Dept Biochem, Singapore 117599, Singapore

[2] Natl Univ Singapore, Yong Loo Lin Sch Med, Ctr Translat Med, Neurosci Res Ctr, Singapore 117599, Singapore

[3] Univ Malaya, Dept Med, Fac Med, Kuala Lumpur 50603, Malaysia

[4] Singapore Gen Hosp, Dept Neurol, Singapore 169608, Singapore

[5] Natl Neurosci Inst, Singapore 169608, Singapore

[6] Khoo Teck Puat Hosp, Dept Med, Singapore 768828, Singapore

[7] Khoo Teck Puat Hosp, Ctr Diabet, Singapore 768828, Singapore

[8] MAHSA Univ Coll, Dept Biochem, Fac Med, Kuala Lumpur 59100, Malaysia

[9] Monash Univ, Fac Med Nursing & Hlth Sci, Dept Anat & Dev Biol, Clayton, Vic 3800, Australia

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2014年 / 15卷 / 01期

基金：

英国医学研究理事会;

关键词：

microRNAs; microarray; cerebral ischemia; brain; diagnosis; blood; TRANSIENT FOCAL ISCHEMIA; EXPRESSION; BLOOD; PLASMA; RNA; DIAGNOSIS; PROFILES; MIR-210; MARKER; BRAIN;

D O I：

10.3390/ijms15011418

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNAs have been identified as key regulators of gene expression and thus their potential in disease diagnostics, prognosis and therapy is being actively pursued. Deregulation of microRNAs in cerebral pathogenesis has been reported to a limited extent in both animal models and human. Due to the complexity of the pathology, identifying stroke specific microRNAs has been a challenge. This study shows that microRNA profiles reflect not only the temporal progression of stroke but also the specific etiologies. A panel of 32 microRNAs, which could differentiate stroke etiologies during acute phase was identified and verified using a customized TaqMan Low Density Array (TLDA). Furthermore we also found 5 microRNAs, miR-125b-2*, -27a*, -422a, -488 and -627 to be consistently altered in acute stroke irrespective of age or severity or confounding metabolic complications. Differential expression of these 5 microRNAs was also observed in rat stroke models. Hence, their specificity to the stroke pathology emphasizes the possibility of developing these microRNAs into accurate and useful tools for diagnosis of stroke.

引用

页码：1418 / 1432

页数：15