Construction and use of PCR primers from a 65 kDa mannoprotein gene for identification of C-albicans

被引：10

作者：

Arancia, S ^{[1
]}

Sandini, S ^{[1
]}

Cassone, A ^{[1
]}

De Bernardis, F ^{[1
]}

La Valle, R ^{[1
]}

机构：

[1] Ist Super Sanita, Dept Infect Parasit & Immune Mediated Dis, I-00161 Rome, Italy

来源：

MOLECULAR AND CELLULAR PROBES | 2004年 / 18卷 / 03期

关键词：

Candida albicans; molecular diagnosis; polymerase chain reaction; CaMP65;

D O I：

10.1016/j.mcp.2003.11.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A method for detection of Candida albicans in biological samples (blood, serum, urine) was developed by the use of polymerase chain reaction (PCR) amplification of a DNA fragment of a gene coding for a 65 kDa mannoprotein of C. albicans (CaMP65). The PCR amplifies a 220 bp fragments whose specificity for C. albicans was demonstrated by Southern blot with a non-radioactive probe, leading to the differentiation from all other yeast species or human and bacterial DNA. The sensitivity of this assay was 5 - 10 C. albicans cells per milliliter of biological sample. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：171 / 175

页数：5

共 25 条

[1]

VAGINOPATHIC AND PROTEOLYTIC CANDIDA SPECIES IN OUTPATIENTS ATTENDING A GYNECOLOGY CLINIC [J].

AGATENSI, L ;

FRANCHI, F ;

MONDELLO, F ;

BEVILACQUA, RL ;

CEDDIA, T ;

DEBERNARDIS, F ;

CASSONE, A .

JOURNAL OF CLINICAL PATHOLOGY, 1991, 44 (10) :826-830

[2]

Construction and use of PCR primers from a 70 kDa heat shock protein gene for identification of Candida albicans [J].

Arancia, S ;

Sandini, S ;

Cassone, A ;

DeBernardis, F ;

LaValle, R .

MOLECULAR AND CELLULAR PROBES, 1997, 11 (05) :329-336

[3]

Armstrong D, 1989, REV INFECT DIS S7, V11, ps1591, DOI [10.1093/clinids/11.Supplement_7.S1591, 10.1093/clinids/11.supplement_7.s1591]

[4]

Semiquantitative polymerase chain reaction enzyme immunoassay for diagnosis of disseminated candidiasis [J].

Burnie, JP ;

Golbang, N ;

Matthews, RC .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1997, 16 (05) :346-350

[5]

USE OF A MONOCLONAL-ANTIBODY IN A DOT-IMMUNOBINDING ASSAY FOR DETECTION OF A CIRCULATING MANNOPROTEIN OF CANDIDA SPP IN NEUTROPENIC PATIENTS WITH INVASIVE CANDIDIASIS [J].

DEBERNARDIS, F ;

GIRMENIA, C ;

BOCCANERA, M ;

ADRIANI, D ;

MARTINO, P ;

CASSONE, A .

JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (12) :3142-3146

[6]

DUPONT B, 1991, FUNGAL INFECT COMPRO, P55

[7]

EPIDEMIOLOGY OF NOSOCOMIAL FUNGAL-INFECTIONS, WITH EMPHASIS ON CANDIDA SPECIES [J].

JARVIS, WR .

CLINICAL INFECTIOUS DISEASES, 1995, 20 (06) :1526-1530

[8]

JONES JM, 1990, CLIN MICROBIOL REV, V3, P779

[9]

POLYMERASE CHAIN-REACTION FOR THE DIAGNOSIS OF CANDIDEMIA [J].

KAN, VL .

JOURNAL OF INFECTIOUS DISEASES, 1993, 168 (03) :779-783

[10]

Generation of a recombinant 65-kilodalton mannoprotein, a major antigen target of cell-mediated immune response to Candida albicans [J].

La Valle, R ;

Sandini, S ;

Gomez, MJ ;

Mondello, F ;

Romagnoli, G ;

Nisini, R ;

Cassone, A .

INFECTION AND IMMUNITY, 2000, 68 (12) :6777-6784

← 1 2 3 →