Improved glutathione production by gene expression in Escherichia coli

被引：41

作者：

Liao, X. -Y.

Shen, W.

Chen, J.

Li, Y.

Du, G. -C. ^{[1
]}

机构：

[1] So Yangtze Univ, Sch Biotechnol, Lab Environm Biotechnol, Wuxi 214036, Peoples R China

[2] So Yangtze Univ, Minist Educ, Key Lab Ind Biotechnol, Wuxi 214036, Peoples R China

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2006年 / 43卷 / 02期

关键词：

Escherichia coli; gene expression; gamma-glutamylcysteine synthetase; glutathione; glutathione synthetase;

D O I：

10.1111/j.1472-765X.2006.01927.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Aims: To improve glutathione (GSH) production in Escherichia coli by different genetic constructions containing GSH genes. methods and Results: GSH production was very low in E. coli by the expression of gshI gene. An increase of GSH production was achieved by the expression of both gshI and gshII genes in E. coli. A higher GSH production, namely 34.8 mg g(-1) wet cell weight, was obtained by simultaneous expression of two copies of gshI gene and one copy of gshII gene. Conclusions: The simultaneous expression of two copies of gshI gene and one copy of gshII gene resulted in a significant increase in GSH production. Significance and Impact of the Study: The expression strategy for GSH production described here can be used to increase gene expression and obtain high production rates in other multienzyme reaction systems.

引用

页码：211 / 214

页数：4

共 19 条

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