Diterpene cyclases responsible for the biosynthesis of phytoalexins, momilactones A, B, and oryzalexins A-F in rice

被引：96

作者：

Otomo, K

Kanno, Y

Motegi, A

Kenmoku, H

Yamane, H

Mitsuhashi, W

Kawa, H

Toshima, H

Itoh, H

Matsuoka, M

Sassa, T

Toyomasu, T ^{[1
]}

机构：

[1] Yamagata Univ, Dept Bioresource Engn, Yamagata 9978555, Japan

[2] Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo 1138657, Japan

[3] Hokkaido Univ, Div Chem, Grad Sch Sci, Sapporo, Hokkaido 0608589, Japan

[4] Ibaraki Univ, Dept Bioresource Sci, Coll Agr, Ibaraki 3000393, Japan

[5] Nagoya Univ, Biosci & Biotechnol Ctr, Nagoya, Aichi 4648601, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2004年 / 68卷 / 09期

关键词：

biosynthesis; diterpene cyclase; momilactone; phytoalexin; rice;

D O I：

10.1271/bbb.68.2001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rice (Oryza sativa L.) produces diterpene phytoalexins, such as momilactones, oryzalexins, and phytocassanes. Using rice genome information and in vitro assay with recombinant enzymes, we identified genes (OsKS4 and OsKS10) encoding the type-A diterpene cyclases 9beta-pimara-7,15-diene synthase and ent-sandaracopimara-diene synthase which are involved in the biosynthesis of momilactones A, B and oryzalexins A-F respectively. Transcript levels of these two genes increased remarkably after ultraviolet (UV) treatment, which is consistent with elevated production of phytoalexins by UV. These two genes might prove powerful tools for understanding plant defense mechanisms in rice.

引用

页码：2001 / 2006

页数：6

共 28 条

[1] NOVEL PHYTOALEXINS (ORYZALEXINS-A, ORYZALEXIN-B AND ORYZALEXIN-C) ISOLATED FROM RICE BLAST LEAVES INFECTED WITH PYRICULARIA-ORYZAE .1. ISOLATION, CHARACTERIZATION AND BIOLOGICAL-ACTIVITIES OF ORYZALEXINS [J].