Quantitative comparisons of in situ microbial biodiversity by signature biomarker analysis

被引:254
作者
White, DC
Stair, JO
Ringelberg, DB
机构
[1] OAK RIDGE NATL LAB,DIV ENVIRONM SCI,OAK RIDGE,TN 37983
[2] UNIV TENNESSEE,DEPT MICROBIOL,KNOXVILLE,TN 37932
来源
JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1996年 / 17卷 / 3-4期
关键词
microbial biodiversity; signature lipid biomarkers (SLB); phospholipid fatty acids (PLFA); in situ microbial biomass; community composition; nutritional/physiological status; non-culturable viable biomass;
D O I
10.1007/BF01574692
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Microscopic examinations have convinced microbial ecologists that the culturable microbes recovered from environmental samples represent a tiny proportion of the extant microbiota. Methods for recovery and enzymatic amplification of nucleic acids from environmental samples have shown that a huge diversity exists in situ, far exceeding any expectations which were based on direct microscopy. It is now theoretically possible to extract, amplify and sequence all the nucleic acids from a community and thereby gain a comprehensive measure of the diversity as well as some insights into the phylogeny of the various elements within this community. Unfortunately, this analysis becomes economically prohibitive if applied to the multitude of niches in a single biome let alone to a diverse set of environments. It is also difficult to utilize PCR amplification on nucleic acids from some biomes because of coextracting enzymatic inhibitors, Signature biomarker analysis which potentially combines gene probe and lipid analysis on the same sample, can serve as a complement to massive environmental genome analysis in providing quantitative comparisons between microniches in the biome under study, This analysis can also give indications of the magnitude of differences in biodiversity in the biome as well as provide insight into the phenotypic activities of each community in a rapid and cost-effective manner, Applications of signature lipid biomarker analysis to define quantitatively the microbial viable biomass of portions of an Eastern USA deciduous forest, are presented.
引用
收藏
页码:185 / 196
页数:12
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