Shed soluble ICAM-1 molecules in bronchoalveolar lavage cell supernatants and serum of patients with pulmonary sarcoidosis

被引:19
作者
Baumer, I [1 ]
Zissel, G [1 ]
Schlaak, M [1 ]
MullerQuernheim, J [1 ]
机构
[1] HOSP MED,RES CTR BORSTEL,D-23845 BORSTEL,GERMANY
关键词
soluble ICAM-1; sarcoidosis; TNF-alpha;
D O I
10.1007/PL00007558
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
The soluble form of intercellular adhesion molecule-1 (sICAM-1) might be a serum parameter of inflammatory activity gauging cellular interactions with possible relevance in sarcoidosis. To address this question we measured sICAM-1 by enzyme-linked immunosorbent assay in serum and shedding of this molecule by bronchoalveolar lavage (BAL) cells in sarcoidosis patients (44 and 40, respectively) and in controls (10 and 19, respectively). Serum concentrations of sICAM-1 (588.3 +/- 72.2 ng/ml) and its spontaneous release by BAL cells (9.9 +/- 1.5 ng/ml) in patients with active sarcoidosis were significantly higher than in those with inactive disease or controls, although no correlation was observed. Significant correlations of sICAM-1 shedding by nonstimulated BAL cells with the serum level of neopterin and of shedding by lipopolysaccharide-stimulated BAL cells with percentage of alveolar macrophages were observed in active sarcoidosis. Kinetic cell culture experiments with peripheral blood mononuclears disclosed a rapid up-regulation of sICAM-1 shedding and tumor necrosis factor-alpha release; however, at 5 h after stimulation a dissociation of their releases was observed. sICAM-1 release was maintained over 2 days, whereas tumor necrosis factor-alpha release peaked at 5 and ceased after 43 h. These results provide evidence that circulating and BAL cell culture-derived sICAM-1 reflect the stage of sarcoid inflammation. Although sICAM-1 in BAL cell supernatants originates from alveolar macrophages; the absence of a correlation with serum sICAM-1 concentration indicates that other cells are additional sources of the circulating pool of this molecule.
引用
收藏
页码:105 / 116
页数:12
相关论文
共 42 条
  • [1] EXPRESSION OF ADHESION MOLECULES (ICAM-1, LFA-3) ON HUMAN EPITHELIAL-CELLS (A549) AFTER RESPIRATORY SYNCYTIAL VIRUS-INFECTION
    ARNOLD, R
    WERCHAU, H
    KONIG, W
    [J]. INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1995, 107 (1-3) : 392 - 393
  • [2] CIRCULATING ICAM-1 LEVELS IN SERUM OF UVEITIS PATIENTS
    AROCKENMETTINGER, E
    STEURERGEORGIEW, L
    STEURER, M
    HUBERSPITZY, V
    HOELZL, E
    GRABNER, G
    KUCHAR, A
    [J]. CURRENT EYE RESEARCH, 1992, 11 : 161 - 166
  • [3] BACHWICH PR, 1986, AM J PATHOL, V125, P421
  • [4] BAUGHMAN RP, 1990, J LAB CLIN MED, V115, P36
  • [5] ENDOTHELIAL-LEUKOCYTE ADHESION MOLECULES IN HUMAN-DISEASE
    BEVILACQUA, MP
    NELSON, RM
    MANNORI, G
    CECCONI, O
    [J]. ANNUAL REVIEW OF MEDICINE, 1994, 45 : 361 - 378
  • [6] DEMONSTRATION OF CELL-SURFACE ANTIGENS AND THEIR ANTIBODIES BY PEROXIDASE-ANTIPEROXIDASE METHOD
    BROSS, KJ
    PANGALIS, GA
    STAATZ, CG
    BLUME, KG
    [J]. TRANSPLANTATION, 1978, 25 (06) : 331 - 334
  • [7] COSTABEL U, 1994, EUR RESPIR J, V7, P624
  • [8] INTERCELLULAR-ADHESION MOLECULE-1 (ICAM-1) IN THE PATHOGENESIS OF MONONUCLEAR CELL ALVEOLITIS IN PULMONARY SARCOIDOSIS
    DALHOFF, K
    BOHNET, S
    BRAUN, J
    KREFT, B
    WIESSMANN, KJ
    [J]. THORAX, 1993, 48 (11) : 1140 - 1144
  • [9] THE FUNCTION OF HUMAN INTERCELLULAR-ADHESION MOLECULE-1 (ICAM-1) IN THE GENERATION OF AN IMMUNE-RESPONSE
    DOUGHERTY, GJ
    MURDOCH, S
    HOGG, N
    [J]. EUROPEAN JOURNAL OF IMMUNOLOGY, 1988, 18 (01) : 35 - 39
  • [10] DUSTIN ML, 1986, J IMMUNOL, V137, P245