Visualization of intracellular trafficking of exogenous DNA delivered by cationic liposomes

被引：43

作者：

Kamiya, H ^{[1
]}

Fujimura, Y ^{[1
]}

Matsuoka, I ^{[1
]}

Harashima, H ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Pharmaceut Sci, Lab Mol Design Pharmaceut, Kita Ku, Sapporo, Hokkaido 0600812, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 298卷 / 04期

关键词：

nonviral vector; cationic liposomes; exogenous DNA; intracellular trafficking; confocal laser microscopy; naked DNA;

D O I：

10.1016/S0006-291X(02)02485-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To visualize the intracellular trafficking of exogenous DNAs delivered by cationic liposomes, rhodamine-labeled DNAs were transfected into NIH3T3 cells and observed by confocal laser microscopy. After 0.5- to 1-h incubations, the DNAs reached the nucleus with a much higher frequency than that expected from the cell division rate. This result suggests that DNAs can enter the nucleus in the presence of the nuclear membrane. Interestingly, some DNAs appeared to extend through the nuclear membrane in the aggregated form which were much larger than the nuclear pore complex. The DNAs which have passed through the nuclear membrane were stained with SYTO 24, a DNA labeling reagent. The stained part may be "naked" DNA that is free of lipids or proteins. This observation indicates that a complex containing DNA fuses with the nuclear membrane and then naked DNA is released into the nucleus. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：591 / 597

页数：7

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