Adherence to stainless steel by foodborne microorganisms during growth in model food systems

被引:207
作者
Hood, SK [1 ]
Zottola, EA [1 ]
机构
[1] UNIV MINNESOTA,DEPT FOOD SCI & NUTR,ST PAUL,MN 55108
关键词
biofilm; adherence; food contact surfaces;
D O I
10.1016/S0168-1605(97)00071-8
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Biofilm formation on stainless steel by Salmonella typhimurium, Listeria monocytogenes, Escherichia coli O157:H7, Pseudomonas fragi and Pseudamonas fluorescens during growth in model food systems was studied. Test growth media included tryptic soy broth (TSB), diluted TSB (dTSB), 1% reconstituted skim milk (RSM) and diluted meat juice (DMJ). Adherent cells were stained with acridine orange and enumerated using epifluorescent microscopy and computerized image analysis. Cells were observed on the stainless steel surface after 1 h in all of the media. However, the increases in the number of adherent cells over time was seen only with S. typhimurium in DMJ, E. coli O157:H7 in TSB, dTSB and DMJ, P. fragi in RSM and P. fluorescens in RSM. The medium which produced the highest observed level of adherent cells was different for each microorganism. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:145 / 153
页数:9
相关论文
共 29 条
[1]  
ALLISON DG, 1987, J GEN MICROBIOL, V133, P1319
[2]  
BEECH IB, 1991, APPL MICROBIOL BIOT, V35, P65, DOI 10.1007/BF00180638
[3]   GROWTH OF BACTERIA AT SURFACES - INFLUENCE OF NUTRIENT LIMITATION [J].
BROWN, CM ;
ELLWOOD, DC ;
HUNTER, JR .
FEMS MICROBIOLOGY LETTERS, 1977, 1 (03) :163-166
[4]   ATTACHMENT OF MICROORGANISMS TO PORK SKIN AND SURFACES OF BEEF AND LAMB CARCASSES [J].
BUTLER, JL ;
STEWART, JC ;
VANDERZANT, C ;
CARPENTER, ZL ;
SMITH, GC .
JOURNAL OF FOOD PROTECTION, 1979, 42 (05) :401-406
[5]   BIOFILMS AND MICROBIAL FOULING [J].
CHARACKLIS, WG ;
COOKSEY, KE .
ADVANCES IN APPLIED MICROBIOLOGY, 1983, 29 :93-138
[6]  
DANKERT J, 1986, CRIT REV BIOCOMPAT, V2, P219
[7]   EXOPOLYSACCHARIDE PRODUCTION IN BIOFILMS - SUBSTRATUM ACTIVATION OF ALGINATE GENE-EXPRESSION BY PSEUDOMONAS-AERUGINOSA [J].
DAVIES, DG ;
CHAKRABARTY, AM ;
GEESEY, GG .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (04) :1181-1186
[8]  
DUNSMORE DG, 1982, AUST J DAIRY TECHNOL, V37, P35
[9]  
ESCHER AR, 1986, COLONIZATION SMOOTH
[10]   EFFECTS OF CULTURE CONCENTRATION AND AGE, TIME, AND TEMPERATURE ON BACTERIAL ATTACHMENT TO POLYSTYRENE [J].
FLETCHER, M .
CANADIAN JOURNAL OF MICROBIOLOGY, 1977, 23 (01) :1-6