Large Structural Change in Isolated Synaptic Vesicles upon Loading with Neurotransmitter

被引：47

作者：

Budzinski, Kristi L. ^{[1
]}

Allen, Richard W. ^{[1
]}

Fujimoto, Bryant S. ^{[1
]}

Kensel-Hammes, P. ^{[2
]}

Belnap, David M. ^{[3
]}

Bajjalieh, Sandra M. ^{[2
]}

Chiu, Daniel T. ^{[1
]}

机构：

[1] Univ Washington, Dept Chem, Seattle, WA 98195 USA

[2] Univ Washington, Dept Pharmacol, Seattle, WA 98195 USA

[3] Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA

来源：

BIOPHYSICAL JOURNAL | 2009年 / 97卷 / 09期

基金：

美国国家科学基金会;

关键词：

VESICULAR GLUTAMATE TRANSPORTER; CORRELATION SPECTROSCOPY; MOTOR PROTEIN; QUANTAL SIZE; RELEASE; INHIBITION; EXOCYTOSIS; DYNAMICS; PRESTIN; STORAGE;

D O I：

10.1016/j.bpj.2009.08.032

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The size of a synaptic vesicle (SV) is generally thought to be determined by the amount of lipid and membrane protein it contains. Once formed, it is thought to remain constant in size. Using fluorescence correlation spectroscopy and cryogenic electron microscopy, we show that glutamatergic vesicles reversibly increase their size upon filling with glutamate. The increase (similar to 25% in diameter) corresponds to an increase in surface area of similar to 50% and in volume of similar to 100%. This large size increase implies a large structural change in the SV upon loading with neurotransmitters. Vesicles lacking SV protein 2A (SV2A) did not manifest a change in size after loading with glutamate, indicating that SV2A is required for this phenomenon.

引用

页码：2577 / 2584

页数：8

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