Enzymatic synthesis of redox-labeled RNA and dual-potential detection at DNA-modified electrodes

被引：51

作者：

Di Giusto, DA

Wlassoff, WA

Giesebrecht, S

Gooding, JJ

King, GC ^{[1
]}

机构：

[1] Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia

[2] Univ New S Wales, Sch Chem Sci, Sydney, NSW 2052, Australia

[3] Inst Cytol & Genet, Novosibirsk 630090, Russia

[4] Univ Bonn, Inst Bot, D-53115 Bonn, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 21期

关键词：

anthraquinones; electrochemistry; ferrocenes; monolayers; RNA polymerase;

D O I：

10.1002/anie.200352977

中图分类号：

O6 [化学];

学科分类号：

0703 [化学];

摘要：

Ferrocene and anthraquinone were used to label RNA (red, dark blue), which could then be detected at an electrode surface bearing complementary DNA oligonucleotide probes (light blue). This approach is an electrochemical analog of popular fluorescence methods for gene expression analysis.

引用

收藏

页码：2809 / 2812

页数：4

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