Measurements of scattered light on a microchip flow cytometer with integrated polymer based optical elements

被引：172

作者：

Wang, Z ^{[1
]}

El-Ali, J ^{[1
]}

Engelund, M ^{[1
]}

Gotsæd, T ^{[1
]}

Perch-Nielsen, IR ^{[1
]}

Mogensen, KB ^{[1
]}

Snakenborg, D ^{[1
]}

Kutter, JP ^{[1
]}

Wolff, A ^{[1
]}

机构：

[1] Tech Univ Denmark, MIC Dept Micro & Nanotechnol, DK-2800 Lyngby, Denmark

来源：

LAB ON A CHIP | 2004年 / 4卷 / 04期

关键词：

D O I：

10.1039/b400663a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Flow cytometry is widely used for analyzing microparticles, such as cells and bacteria. In this paper, we report an innovative microsystem, in which several different optical elements (waveguides, lens and fiber-to-waveguide couplers) are integrated with microfluidic channels to form a complete microchip flow cytometer. All the optical elements, the microfluidic system, and the fiber-to-waveguide couplers were defined in one layer of polymer (SU-8, negative photoresist) by standard photolithography. With only a single mask procedure required, all the fabrication and packaging processes can be finished in one day. Polystyrene beads were measured in the microchip flow cytometer, and three signals (forward scattering, large angle scattering and extinction) were measured simultaneously for each bead. To our knowledge this is the first time forward scattered light and incident light extinction were measured in a microsystem using integrated optics. The microsystem can be applied for analyzing different kinds of particles and cells, and can easily be integrated with other microfluidic components.

引用

页码：372 / 377

页数：6

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