Potent hydroxyl radical-scavenging activity of drought-induced type-2 metallothionein in wild watermelon

被引：159

作者：

Akashi, K ^{[1
]}

Nishimura, N ^{[1
]}

Ishida, Y ^{[1
]}

Yokota, A ^{[1
]}

机构：

[1] Nara Inst Sci & Technol, Grad Sch Biol Sci, Nara 6300101, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 323卷 / 01期

关键词：

metallothionein; wild watermelon; drought tolerance; hydroxyl radical; oxidative stress;

D O I：

10.1016/j.bbrc.2004.08.056

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Wild watermelon (Citrullus lanatus sp.) has the ability to tolerate severe drought/high light stress conditions despite carrying out normal C-3-type photosynthesis. Here, mRNA differential display was employed to isolate drought-responsive genes in the leaves of wild watermelon. One of the isolated genes, CLMT2. shared significant homology with type-2 metallothionein (MT) sequences from other plants. The second-order rate constant for the reaction between a recombinant CLMT2 protein and hydroxyl radicals was estimated to be 1.2 x 10(11) M(-1)s(-1), demonstrating that CLMT2 had an extraordinary high activity for detoxifying hydroxyl radicals. Moreover, hydroxyl radical-catalyzed degradation of watermelon genomic DNA was effectively suppressed by CLMT2 in vitro. This is the first demonstration of a plant MT with antioxidant properties. The results suggest that CLMT2 induction contributes to the survival of wild watermelon under severe drought/high light stress conditions. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：72 / 78

页数：7

共 43 条

[1] Citrulline, a novel compatible solute in drought-tolerant wild watermelon leaves, is an efficient hydroxyl radical scavenger [J].

Akashi, K ;

Miyake, C ;

Yokota, A .

FEBS LETTERS, 2001, 508 (03) :438-442

[2] A COMPILATION OF SPECIFIC BIMOLECULAR RATE CONSTANTS FOR REACTIONS OF HYDRATED ELECTRONS HYDROGEN ATOMS AND HYDROXYL RADICALS WITH INORGANIC AND ORGANIC COMPOUNDS IN AQUEOUS SOLUTION [J].

ANBAR, M ;

NETA, P .

INTERNATIONAL JOURNAL OF APPLIED RADIATION AND ISOTOPES, 1967, 18 (07) :493-&

[3] The water-water cycle in chloroplasts: Scavenging of active oxygens and dissipation of excess photons [J].

Asada, K .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1999, 50 :601-639

[4]

Ausubel F.M., 1994, CURRENT PROTOCOLS MO

[5] PLANT PRODUCTIVITY AND ENVIRONMENT [J].

BOYER, JS .

SCIENCE, 1982, 218 (4571) :443-448

[6]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[7] ISOLATION OF CDNA CLONES FOR GENES THAT ARE EXPRESSED DURING LEAF SENESCENCE IN BRASSICA-NAPUS - IDENTIFICATION OF A GENE ENCODING A SENESCENCE-SPECIFIC METALLOTHIONEIN-LIKE PROTEIN [J].

BUCHANANWOLLASTON, V .

PLANT PHYSIOLOGY, 1994, 105 (03) :839-846

[8] Molecular cloning of a metallothionein-like gene from Nicotiana glutinosa L and its induction by wounding and tobacco mosaic virus infection [J].

Choi, D ;

Kim, HM ;

Yun, HK ;

Park, JA ;

Kim, WT ;

Bok, SH .

PLANT PHYSIOLOGY, 1996, 112 (01) :353-359

[9] METALLOTHIONEIN PROTECTS DNA FROM OXIDATIVE DAMAGE [J].

CHUBATSU, LS ;

MENEGHINI, R .

BIOCHEMICAL JOURNAL, 1993, 291 :193-198

[10]

COUPE SA, 1995, PLANTA, V197, P442, DOI 10.1007/BF00196665

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