Potent hydroxyl radical-scavenging activity of drought-induced type-2 metallothionein in wild watermelon

被引：159

作者：

Akashi, K ^{[1
]}

Nishimura, N ^{[1
]}

Ishida, Y ^{[1
]}

Yokota, A ^{[1
]}

机构：

[1] Nara Inst Sci & Technol, Grad Sch Biol Sci, Nara 6300101, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 323卷 / 01期

关键词：

metallothionein; wild watermelon; drought tolerance; hydroxyl radical; oxidative stress;

D O I：

10.1016/j.bbrc.2004.08.056

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Wild watermelon (Citrullus lanatus sp.) has the ability to tolerate severe drought/high light stress conditions despite carrying out normal C-3-type photosynthesis. Here, mRNA differential display was employed to isolate drought-responsive genes in the leaves of wild watermelon. One of the isolated genes, CLMT2. shared significant homology with type-2 metallothionein (MT) sequences from other plants. The second-order rate constant for the reaction between a recombinant CLMT2 protein and hydroxyl radicals was estimated to be 1.2 x 10(11) M(-1)s(-1), demonstrating that CLMT2 had an extraordinary high activity for detoxifying hydroxyl radicals. Moreover, hydroxyl radical-catalyzed degradation of watermelon genomic DNA was effectively suppressed by CLMT2 in vitro. This is the first demonstration of a plant MT with antioxidant properties. The results suggest that CLMT2 induction contributes to the survival of wild watermelon under severe drought/high light stress conditions. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：72 / 78

页数：7

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