Comparative study of transfection efficiency of cationic cholesterols mediated by liposomes-based gene delivery

被引：38

作者：

Hasegawa, S ^{[1
]}

Hirashima, N ^{[1
]}

Nakanishi, M ^{[1
]}

机构：

[1] Nagoya City Univ, Grad Sch Pharmaceut Sci, Mizuho Ku, Nagoya, Aichi 4678603, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2002年 / 12卷 / 09期

关键词：

D O I：

10.1016/S0960-894X(02)00119-1

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

To develop the efficient non-viral vector for gene delivery, we compared transfection activities of cationic cholesterol derivatives. We found that the stability of the liposome-DNA complex in the presence of endosome deeply related to the transfection efficiency. We also found that the introduction of a hydrophilic group to the amino terminal of the cholesterol derivative decreased stability and facilitated the release of DNA from the endosome, resulting in higher transfection efficiency. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：1299 / 1302

页数：4

共 14 条

[1]

AUSTIN LB, 1997, BIOCHEMISTRY-US, V36, P1628

[2] Synthesis, activity, and structure-activity relationship studies of novel cationic lipids for DNA transfer [J].

Byk, G ;

Dubertret, C ;

Escriou, V ;

Frederic, M ;

Jaslin, G ;

Rangara, R ;

Pitard, B ;

Crouzet, J ;

Wils, P ;

Schwartz, B ;

Scherman, D .

JOURNAL OF MEDICINAL CHEMISTRY, 1998, 41 (02) :224-235

[3] EFFECT OF CATIONIC CHOLESTEROL DERIVATIVES ON GENE-TRANSFER AND PROTEIN-KINASE-C ACTIVITY [J].

FARHOOD, H ;

BOTTEGA, R ;

EPAND, RM ;

HUANG, L .

BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1111 (02) :239-246

[4] LIPOFECTION - A HIGHLY EFFICIENT, LIPID-MEDIATED DNA-TRANSFECTION PROCEDURE [J].

FELGNER, PL ;

GADEK, TR ;

HOLM, M ;

ROMAN, R ;

CHAN, HW ;

WENZ, M ;

NORTHROP, JP ;

RINGOLD, GM ;

DANIELSEN, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (21) :7413-7417

[5] Gene transfection activities of amphiphilic steroid-polyamine conjugates [J].

Fujiwara, T ;

Hasegawa, S ;

Hirashima, N ;

Nakanishi, M ;

Ohwada, T .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2000, 1468 (1-2) :396-402

[6] A NOVEL CATIONIC LIPOSOME REAGENT FOR EFFICIENT TRANSFECTION OF MAMMALIAN-CELLS [J].

GAO, X ;

HUANG, L .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 179 (01) :280-285

[7] Nature of linkage between the cationic headgroup and cholesteryl skeleton controls gene transfection efficiency [J].

Ghosh, YK ;

Visweswariah, SS ;

Bhattacharya, S .

FEBS LETTERS, 2000, 473 (03) :341-344

[8] Microtubule involvement in the intracellular dynamics for gene transfection mediated by cationic liposomes [J].

Hasegawa, S ;

Hirashima, N ;

Nakanishi, M .

GENE THERAPY, 2001, 8 (21) :1669-1673

[9] Novel applications of liposomes [J].

Lasic, DD .

TRENDS IN BIOTECHNOLOGY, 1998, 16 (07) :307-321

[10] Membrane fusion plays an important role in gene transfection mediated by cationic liposomes [J].

Noguchi, A ;

Furuno, T ;

Kawaura, C ;

Nakanishi, M .

FEBS LETTERS, 1998, 433 (1-2) :169-173

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